Background. Osteosarcoma (OS) is the most frequent bone tumor with high metastasis. This study is aimed at assessing the expression and prognostic significance of microRNA-1826 (miR-1826) in OS patients, as well as its biological function in tumor progression. Methods. Quantitative Real-Time PCR was employed to measure the expression of miR-1826 in OS tissues and cell lines. Kaplan-Meier survival analysis and Cox regression model were used to evaluate the prognostic value of miR-1826. CCK-8 and Transwell assay were conducted to investigate the effect of miR-1826 on OS cell proliferation, migration, and invasion. Results. miR-1826 expression was downregulated in OS tissues and cell lines and associated with OS patients’ clinical stage and distant metastasis. Low levels of miR-1826 were related with shorter survival time and determined as an independent prognostic indicator for the overall survival of OS patients. The overexpression of miR-1826 in OS cells led to inhibited cell proliferation, migration, and invasion. Conclusion. The decreased expression of miR-1826 predicts a poor prognosis in OS patients, and its overexpression inhibits OS cell proliferation, migration, and invasion. This newly identified miR-1826 provides a novel sight into the pathogenesis of OS and offers a candidate prognostic biomarker and therapeutic target for OS treatment.

中文翻译：

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miR-1826 的下调表明骨肉瘤患者的预后不良并调节肿瘤细胞的增殖、迁移和侵袭。

背景。骨肉瘤（OS）是最常见的高转移骨肿瘤。本研究旨在评估 microRNA-1826 (miR-1826) 在 OS 患者中的表达和预后意义，以及其在肿瘤进展中的生物学功能。方法。采用定量实时 PCR 来测量 miR-1826 在 OS 组织和细胞系中的表达。Kaplan-Meier生存分析和Cox回归模型用于评估miR-1826的预后价值。进行 CCK-8 和 Transwell 测定以研究 miR-1826 对 OS 细胞增殖、迁移和侵袭的影响。结果. miR-1826 在 OS 组织和细胞系中的表达下调，并与 OS 患者的临床分期和远处转移有关。低水平的 miR-1826 与较短的生存时间相关，并被确定为 OS 患者总体生存的独立预后指标。miR-1826 在 OS 细胞中的过表达导致抑制细胞增殖、迁移和侵袭。结论。miR-1826 表达降低预示着 OS 患者预后不良，其过表达抑制 OS 细胞增殖、迁移和侵袭。这种新发现的 miR-1826 为 OS 的发病机制提供了新的视角，并为 OS 治疗提供了候选的预后生物标志物和治疗靶点。