Salt processing, which involves steaming with salt water, directs herbs into the kidney channel. After being salt processed, kidney invigorating effects occur. However, the underlying mechanism of this method remains elusive. The compounds monotropein, rubiadin, and rubiadin 1-methyl ether are the major effective components of Morinda officinalis How. To clarify the pharmacokinetics and tissue distribution of these three compounds, we employed liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) to determine the contents of the three components in rat plasma and tissues. Separation was achieved on an Acquity UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm, Waters). Formic acid aqueous solution (0.1%; A) and acetonitrile (containing 0.1% formic acid; B) were used as the mobile phase system with a programmed elution of 0∼5 min with 70% A and then 5∼7 min with 60% A. All analytes were measured with optimized multiple reaction monitoring (MRM) in negative ion mode. Geniposide and 1,8-dihydroxyanthraquinone were used as the internal standards (IS). The linear ranges were 1.2∼190, 1.3∼510, and 0.047∼37.5 μg/mL, respectively. Compared with the Morinda officinalis without wood (MO) group, the Cmax and AUC0-t parameters of rubiadin and rubiadin 1-methyl ether elevated remarkably for the salt-processed Morinda officinalis (SMO) groups, which indicates that steaming by salt could increase the bioavailability of rubiadin and rubiadin 1-methyl ether. The Tmax for monotropein is shorter (0.5 h) in SMO groups than that in MO group, which means that monotropein was quickly absorbed in the SMO extract. Moreover, the contents of three compounds in the small intestine were the highest.

中文翻译：

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盐处理方法对口服巴戟天的药代动力学和组织分布的影响。提取。

盐处理涉及用盐水蒸煮，将草药导入肾脏通道。经过盐处理后，会产生补肾的作用。但是，此方法的基本机制仍然难以捉摸。化合物monotropein，茜草素和茜草素1-甲基醚是巴戟天的主要有效成分。为了阐明这三种化合物的药代动力学和组织分布，我们采用液相色谱-串联质谱法（UPLC-MS / MS）测定大鼠血浆和组织中这三种成分的含量。在Acquity UPLC HSS T3色谱柱（100 mm×2.1 mm，1.8μm，Waters）上进行分离。甲酸水溶液（0.1％; A）和乙腈（含0.1％甲酸; B）用作流动相系统，程序设定为70％A的0〜5分钟洗脱，然后60％A的5〜7分钟洗脱。所有分析物均以负离子模式通过优化的多反应监测（MRM）进行测量。ip子苷和1,8-二羟基蒽醌用作内标（IS）。线性范围分别为1.2〜190、1.3〜510和0.047〜37.5μg/ mL。盐处理的巴戟天（SMO）组的茜草素和茜草素1-甲基醚的Cmax和AUC0-t参数与无木巴戟天（MO）组相比，表明通过盐蒸可以增加茜草素和茜草素1-甲基醚的生物利用度。在SMO组中，相对于MO组，单糖足蛋白的Tmax较短（0.5 h），这意味着单糖足蛋白被SMO提取物快速吸收。