Polymerase chain reaction followed by high resolution melting (PCR-HRM) analysis is a simple, rapid and accurate method for molecular detection of various nematode species. The objective of the present study was, for the first time, to develop a PCR-HRM assay for the detection of various animal Trichostrongylus spp. A pair of primers targeting the ITS-2 rDNA region of the Trichostrongylus spp. was designed for the development of the HRM assay. DNA samples were extracted from 30 adult worms of Trichostrongylus spp., the ITS-2-rDNA region was amplified using PCR, and the resultant products were sequenced and characterized. Afterwards, the PCR-HRM analysis was conducted to detect and discriminate Trichostrongylus spp. Molecular sequence analysis revealed that 24, 4, and 1 of the samples were T. colubriformis, T. vitrinus and T. capricola, respectively. Results from PCR-HRM indicated that complete agreement was relatively found between speciation by HRM analysis and DNA sequencing for the detection of Trichostrongylus species. The PCR-HRM analysis method developed in the present study is fast and low-cost; the method can be comparable with other molecular detection techniques, representing a reliable tool for the identification of various species within the Trichostrongylus genus.

聚合酶链反应后进行高分辨率熔解（PCR-HRM）分析是一种简单，快速，准确的分子检测各种线虫物种的方法。本研究的目的是首次开发一种PCR-HRM分析方法，用于检测各种动物的Trichostrongylus spp。靶向Trichostrongylus spp的ITS-2 rDNA区域的一对引物。设计用于开发HRM分析。从30种毛线虫的成虫中提取DNA样品，使用PCR扩增ITS-2-rDNA区域，并对所得产物进行测序和鉴定。之后，进行PCR-HRM分析以检测和区分毛圆线虫spp。分子序列分析表明，24，如图4所示，并且将样品的1是T.蛇形毛圆线虫，T. vitrinus和T. capricola，分别。PCR-HRM的结果表明，在通过HRM分析进行的物种形成和DNA测序中检测毛圆线虫物种方面，完全可以找到完全一致的协议。本研究开发的PCR-HRM分析方法快速，低成本。该方法可以与其他分子检测技术相提并论，代表了一种用于鉴定毛圆线虫属内各种物种的可靠工具。