In our previous study, we found that the methanolic extract of Sanoshashinto (三黄瀉心湯) (SHXXTM) exhibited significant vasorelaxant effects in vitro and antihypertensive effects in vivo, and baicalin and berberine were the main antihypertensive constituents in SHXXTM. We also speculated that the baicalin–berberine (BB) combination produced vasorelaxant effects by activating the NO/cGMP pathway, and the BK_Ca channel and the DAG/PKC/CPI-17 pathway were involved. In this study, we examined the vasorelaxant effects using helical strips of rat aorta pretreated with different activators or inhibitors. The results suggested that the K_ATP channel and the voltage-dependent Ca²⁺ channel (VDCC) were also involved in the vasorelaxant effects. Furthermore, we found that SHXXTM and the BB combination reduced left ventricular hypertrophy and altered gut microbiota. Together, the results indicated that Sanoshashinto might have comprehensive effects on ameliorating hypertension.

中文翻译：

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Sanoshashinto对自发性高血压大鼠左室肥大和肠道菌群的影响。

在我们先前的研究中，我们发现三黄泻心汤（SHXXTM）的甲醇提取物在体外具有明显的血管舒张作用，在体内具有降压作用，而黄ba苷和小ber碱是SHXXTM中主要的降压成分。我们还推测，黄ical素与小ber碱（BB）组合通过激活NO / cGMP途径产生血管舒张作用，并且涉及BK _Ca通道和DAG / PKC / CPI-17途径。在这项研究中，我们检查了使用不同激活剂或抑制剂预处理的大鼠主动脉螺旋带对血管松弛的作用。结果表明，K _ATP通道和电压依赖性Ca ²⁺通道（VDCC）也参与血管舒张作用。此外，我们发现SHXXTM和BB组合可减少左心室肥大并改变肠道菌群。总之，结果表明Sanoshashinto可能对减轻高血压具有综合作用。