• In many seed plants, perennialism is achieved through axillary buds and side shoots that remain vegetative. This work aimed to analyse the pattern of axillary bud (AB) formation in the perennial model plant Arabis alpina and to study the role of the LATERAL SUPPRESSOR (AaLAS ) gene.
• This study combines stereomicroscopic analysis with RNA sequencing to monitor the correlation between patterns of AB formation and gene expression. The role of AaLAS was studied using an RNA interference (RNAi) approach.
• During vegetative development, ABs initiate at a distance from the shoot apical meristem (SAM), whereas after floral induction, they initiate adjacent to the SAM. Dormant buds are established before the onset of vernalization. Transcript profiles of ABs initiated at a distance differed from those in the SAM, whereas those of buds initiated in close proximity were similar. Knockdown of AaLAS leads to the loss of dormant buds and vegetative side shoots, strongly compromising the perennial life cycle.
• AB formation is regulated differently during vegetative and reproductive development. New meristems that possess different gene expression profiles from those in the SAM are established at a distance from the SAM. AaLAS is essential for the perennial life cycle by modulating the establishment of dormant buds and vegetative side shoots.

中文翻译：

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保持不同的距离：腋芽在离芽顶分生组织一定距离处萌发，对于阿拉比斯高山白菜的多年生生活方式至关重要。

• 在许多种子植物中，多年生植物是通过保持营养的腋芽和侧枝实现的。这项工作的目的是分析常年模式植物腋芽（AB）形成的图案高山南芥和研究的作用侧向抑制器（AaLAS）基因。
• 这项研究将立体显微镜分析与RNA测序相结合，以监测AB形成模式与基因表达之间的相关性。使用RNA干扰（RNAi）方法研究了AaLAS的作用。
• 在营养发育过程中，AB距茎尖分生组织（SAM）较远，而在花诱导后，它们在SAM附近起始。休眠芽在春化开始之前就已建立。在远处启动的AB的转录物谱与在SAM中不同，而近距离启动的芽的转录物谱相似。击倒AaLAS会导致休眠芽和营养侧枝的丢失，从而极大地损害了多年生的生命周期。
• 营养生长和生殖发育期间，AB的形成受到不同的调节。在距SAM一定距离处建立具有与SAM中不同的基因表达谱的新分生组织。通过调节休眠芽和无性侧芽的建立，AaLAS对于多年生生命周期至关重要。