This review describes the premises for accurate measurement of the gut hormone and satiety factor cholecystokinin (CCK) in circulation. Such a description is useful for nutrition and obesity research in which CCK in its satiety role has evoked considerable interest during the last decades. The background for the review is two sorts of considerations or concerns. First, CCK is a complex peptide system that in several ways challenges plasma measurements because the concentrations in plasma are very low (in the femtomolar to low picomolar range), and the bioactive CCK circulates in different molecular forms (CCK-58, -33, -22, and -8). Furthermore, there are major specificity problems because the structurally similar gastrin hormone circulates in 10- to 20-fold higher concentrations, and in addition, plasma proteins may, due to their high concentration, interfere in an unspecific way with immunoassay measurements. The second concern is that several obesity studies in recent decades have been based on commercial CCK kits with often inadequate documentation of the reliability in plasma measurement. Consequently, many plasma CCK results in today's obesity studies are difficult to compare. Moreover, the use of even fairly reliable commercial CCK kits has recently suffered from sudden discontinuation of the kit production, which has endangered several projects in nutrition and obesity research.

中文翻译：

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参考营养相关肥胖研究测量血浆中的胆囊收缩素

这篇综述描述了准确测量循环中肠道激素和饱腹感因子胆囊收缩素 (CCK) 的前提。这种描述对于营养和肥胖研究很有用，在过去的几十年中，CCK 在其饱腹感方面的作用引起了相当大的兴趣。审查的背景是两种考虑或关注。首先，CCK 是一个复杂的肽系统，它在多种方面挑战血浆测量，因为血浆中的浓度非常低（在飞摩尔到低皮摩尔范围内），并且生物活性 CCK 以不同的分子形式（CCK-58、-33、 -22 和 -8）。此外，存在主要的特异性问题，因为结构相似的胃泌素激素以高 10 到 20 倍的浓度循环，此外，血浆蛋白可能，由于它们的高浓度，以非特异性方式干扰免疫测定测量。第二个担忧是，近几十年来的几项肥胖研究都是基于商业 CCK 试剂盒，而血浆测量可靠性的文件往往不充分。因此，当今肥胖研究中的许多血浆 CCK 结果难以比较。此外，即使是相当可靠的商业 CCK 试剂盒的使用最近也遭受了试剂盒生产的突然中断，这已经危及营养和肥胖研究中的几个项目。许多血浆 CCK 结果在当今的肥胖研究中难以比较。此外，即使是相当可靠的商业 CCK 试剂盒的使用最近也遭受了试剂盒生产的突然中断，这已经危及营养和肥胖研究中的几个项目。许多血浆 CCK 结果在当今的肥胖研究中难以比较。此外，即使是相当可靠的商业 CCK 试剂盒的使用最近也遭受了试剂盒生产的突然中断，这已经危及营养和肥胖研究中的几个项目。