The objective of this research was to study a novel ozone-air flotation microalgae harvesting method and evaluate its effect on the recovery of biomass and biocomponents (lipids, carbohydrates, proteins). Best processing conditions were established using a response surface methodology (RSM). Microalgae separation and biocomponent recovery were evaluated according to changes in gas concentration (13, 18 and 25 mgO3/L), ozone dose (0.04, 0.09 and 0.16 mg O3/mg biomass) and airflow rate (0.5, 1.0 and 1.5 L/min). More than 95% of the biomass was recovered from wastewater at an ozone-air combination of 0.09 mgO3/mg biomass and 1.5 L air/min. Using ozone-air represented a reduction of 59% in the ozone dose compared to the flotation process solely using ozone (0.22 mgO3/mg biomass). In addition, there was an improved yield in the recovery of all microalgae biocomponents. A maximum yield of 0.18 mg lipids/mg biomass was achieved at: 0.16 mg O3/mg biomass, 25 mg gas O3/L and 1.5 L air/min. In conclusion, combining the use of ozone-air for separation of microalgae reduces ozone requirement and enhances lipids and proteins post-extraction.

中文翻译：

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使用臭氧气浮收集微藻以回收生物质、脂质、碳水化合物和蛋白质。

本研究的目的是研究一种新的臭氧气浮微藻收获方法，并评估其对生物质和生物成分（脂质、碳水化合物、蛋白质）回收的影响。使用响应面方法 (RSM) 建立最佳加工条件。根据气体浓度（13、18 和 25 mgO3/L）、臭氧剂量（0.04、0.09 和 0.16 mg O3/mg 生物量）和气流速率（0.5、1.0 和 1.5 L/min）的变化评估微藻分离和生物成分回收）。在 0.09 mgO3/mg 生物质和 1.5 L 空气/分钟的臭氧-空气组合下，超过 95% 的生物质从废水中回收。与仅使用臭氧（0.22 mgO3/mg 生物量）的浮选工艺相比，使用臭氧空气代表臭氧剂量减少了 59%。此外，所有微藻生物成分的回收率均有所提高。在 0.16 mg O3/mg 生物质、25 mg 气体 O3/L 和 1.5 L 空气/分钟下实现了 0.18 mg 脂质/mg 生物质的最大产量。总之，结合使用臭氧-空气分离微藻可减少臭氧需求并增强提取后的脂质和蛋白质。