Klebsiella pneumoniae is an important pathogen that causes pneumonia and bloodstream infections, especially in neonates and intensive care patients. The carbapenems remain an important therapeutic option for clinicians, particularly against cephalosporin-resistant Gram-negative pathogens. This increased use of carbapenems at clinics has resulted in the evolution and spread of carbapenem-resistant K. pneumoniae. In this study, we isolated six bla K. pneumoniae carbapenemase (KPC)-producing strains belonging to sequence type 258 (ST258) from clinical, environmental, and veterinary sources. Antibiotic susceptibility was performed on these isolates and the genes responsible for extended-spectrum beta-lactamases and metallo-beta-lactamase production were screened. The molecular typing was done using multilocus sequence typing. Isolated strains were resistant to various antibiotic classes, including carbapenems, and carried the carbapenem-resistant gene, blaKPC. All strains were susceptible to tigecycline and colistin. This is the first report detecting K. pneumoniae ST258 strains in Pakistan.

中文翻译：

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在巴基斯坦分离出的第一株blaKPC携带肺炎克雷伯菌ST258菌株。

肺炎克雷伯菌是引起肺炎和血液感染的重要病原体，尤其是在新生儿和重症监护患者中。碳青霉烯类仍然是临床医生的重要治疗选择，尤其是对抗头孢菌素耐药的革兰氏阴性病原体。在临床上碳青霉烯类药物的使用增加，导致了对碳青霉烯类耐药的肺炎克雷伯菌的进化和传播。在这项研究中，我们分离了六个bla肺炎克雷伯菌来源于临床，环境和兽医学的序列类型258（ST258）的碳青霉烯酶（KPC）生产菌株。对这些分离物进行了抗生素敏感性分析，并筛选了导致广谱β-内酰胺酶和金属-β-内酰胺酶产生的基因。使用多基因座序列分型进行分子分型。分离的菌株对包括碳青霉烯类在内的各种抗生素具有抗性，并带有抗碳青霉烯类基因blaKPC。所有菌株均对替加环素和粘菌素敏感。这是在巴基斯坦检测到肺炎克雷伯菌ST258菌株的第一份报告。