Excretory/secretory proteins of Haemonchus contortus (HcESPs) intermingle comprehensively with host immune cells and modulate host immune responses. In this study, H contortus ES antigen named as elongation factor 1 alpha (HcEF-1α) was cloned and expressed. The influences of recombinant HcEF-1α on multiple functions of goat peripheral blood mononuclear cells (PBMCs) were observed in vitro. Immunoblot analysis revealed that rHcEF-1α was recognized by the serum of goat infected with H contortus. Immunofluorescence analysis indicated that rHcEF-1α was bound on surface of PBMCs. Moreover, the productions of IL-4, TGF-β1, IFN-γ and IL-17 of cells were significantly modulated by the incubation with rHcEF-1α. The production of interleukin IL-10 was decreased. Cell migration, cell proliferation and cell apoptosis were significantly increased; however, nitric oxide production (NO) was significantly decreased. The MHC II molecule expression of cells incubated with rHcEF-1α was increased significantly, whereas MHC-I was not changed as compared to the control groups (PBS control and pET32a). These findings indicated that rHcEF-1α protein might play essential roles in functional regulations of HcESPs on goat PBMC and mediate the immune responses of the host during host-parasite relationship.

中文翻译：

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捻转血矛线虫的重组伸长因子1α影响山羊PBMC的功能。

捻转血矛线虫（HcESPs）的排泄/分泌蛋白与宿主免疫细胞全面融合并调节宿主免疫反应。在本研究中，克隆并表达了称为延伸因子1α（HcEF-1α）的H contortus ES抗原。体外观察到重组HcEF-1α对山羊外周血单个核细胞（PBMCs）多种功能的影响。免疫印迹分析表明，rHcEF-1α被感染了H.Contortus的山羊血清识别。免疫荧光分析表明rHcEF-1α结合在PBMC的表面。此外，通过与rHcEF-1α一起温育显着调节细胞的IL-4，TGF-β1，IFN-γ和IL-17的产生。白介素IL-10的产生减少。细胞迁移，细胞增殖和细胞凋亡明显增加；然而，一氧化氮的产生（NO）明显降低。与对照组（PBS对照和pET32a）相比，与rHcEF-1α孵育的细胞的MHC II分子表达显着增加，而MHC-1没有变化。这些发现表明，rHcEF-1α蛋白可能在山羊PBMC上HcESPs的功能调节中起重要作用，并在宿主与寄生虫的关系中介导宿主的免疫反应。