Phenotypic variation can arise from differences in the protein coding sequence and in the regulatory elements. However, little is known about the contribution of regulatory difference to the expression divergence, especially the cis and trans regulatory variation to the expression divergence in intraspecific populations. In this study, we used two different yeast strains, BY4743 and RM11‐1a/α, to study the regulatory variation to the expression divergence between BY and RM under oxidative stress condition. Our results indicated that the expression divergence of BY and RM is mainly due to trans regulatory variations under both normal and oxidative stress conditions. However, cis regulatory variation seems to play a very important role in oxidative stress response in yeast because 36% of genes showed an increase in cis regulatory variation effect compared with 13% of genes that showed an increase in trans regulatory variation effect after oxidative stress. Our data also indicated that genes located on the longer arm of the chromosomes are more susceptible to cis variation effect under oxidative stress than genes on the shorter arm of the chromosomes.

中文翻译：

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基因位置对种内酵母氧化反应基因表达差异的影响

表型变异可能源于蛋白质编码序列和调控元件的差异。然而，关于调控差异对表达差异的贡献知之甚少，尤其是顺式和反式调控变异对种内种群表达差异的影响。在本研究中，我们使用两种不同的酵母菌株 BY4743 和 RM11-1a/α，研究氧化应激条件下 BY 和 RM 表达差异的调节变异。我们的结果表明，BY 和 RM 的表达差异主要是由于正常和氧化应激条件下的反式调节变化。然而，顺式调控变异似乎在酵母的氧化应激反应中起着非常重要的作用，因为在氧化应激后，36% 的基因显示出顺式调控变异效应增加，而 13% 的基因显示出反式调控变异效应增加。我们的数据还表明，在氧化应激下，位于染色体较长臂上的基因比位于染色体较短臂上的基因更容易受到顺式变异效应的影响。