The present study was aimed to develop a membrane sparger (MS) integrated into a tubular photobioreactor to promote the increase of the carbon dioxide (CO₂) fixation by Spirulina sp. LEB 18 cultures. The use of MS for the CO₂ supply in Spirulina cultures resulted not only in the increase of DIC concentrations but also in the highest accumulated DIC concentration in the liquid medium (127.4 mg L⁻¹ d⁻¹). The highest values of biomass concentration (1.98 g L⁻¹), biomass productivity (131.8 mg L⁻¹ d⁻¹), carbon in biomass (47.9% w w⁻¹), CO₂ fixation rate (231.6 mg L⁻¹ d⁻¹), and CO₂ use efficiency (80.5% w w⁻¹) by Spirulina were verified with MS, compared to the culture with conventional sparger for CO₂ supply. Spirulina biomass in both culture conditions had high protein contents varying from 64.9 to 69% (w w⁻¹). MS can be considered an innovative system for the supply of carbon for the microalgae cultivation and biomass production. Moreover, the use of membrane system might contribute to increased process efficiency with a reduced cost of biomass production.

中文翻译：

---

微藻培养中二氧化碳供应的膜分布器的创新开发。

本研究旨在开发一种集成到管式光生物反应器中的膜分布器 (MS)，以促进螺旋藻对二氧化碳 (CO ₂ ) 的固定。LEB 18 文化。在螺旋藻培养物中使用 MS供应CO ₂不仅导致 DIC 浓度增加，而且导致液体培养基中累积的最高 DIC 浓度（127.4 mg L ^-1 d ^-1）。生物质浓度 (1.98 g L ^-1 )、生物质生产力 (131.8 mg L ^-1 d ^-1 )、生物质中的碳 (47.9% ww ^-1 )、CO ₂的最高值与使用传统喷雾器供应CO ₂的培养物相比，螺旋藻的固定率（231.6 mg L ^-1 d ^-1）和 CO ₂使用效率（80.5% ww ^-1）通过MS 进行了验证。两种培养条件下的螺旋藻生物质都具有高蛋白质含量，从 64.9% 到 69% (ww ^-1 )。MS 可以被认为是一种为微藻培养和生物质生产提供碳的创新系统。此外，膜系统的使用可能有助于提高工艺效率，同时降低生物质生产的成本。