Although tea seed cake (TSC) possesses high nutritional value, its high content of tea saponin (TS) limits its potential as feed. This study aimed to degrade TS in TSC by saponin-degrading strain and used a multistrains fermentation method to improve its nutritional value and palatability. Three saponin-degrading strains were isolated from Oleum Camelliae mill soil and identified as Citrobacter sp. FCTS301, Pantoea sp. FCTS302, and Enterobacter sp. FCTS303. Single-factor experiment showed that Citrobacter sp. FCTS301 had the highest degradation rate of TS. Response surface analysis for Citrobacter sp. FCTS301 indicated that the optimum culture conditions were as follows: initial pH of 7.2, culture temperature of 34.2 °C, inoculation amount of 7.3%, the agitation rate of 150 rpm, and the TS concentration of 10.0 g/L. Under these conditions, the maximum degradation rate was 82.6%. The fermentation process of TSC was obtained by a multistrains fermentation experiment. Considering the protein content, crude fiber degradation rate, and TS degradation rate of each group, the optimum inoculum amount of strains included Citrobacter sp. FCTS301, Aspergillus oryzae NCUF414, Saccharomyces cersvisiae NCUF306.5, and Lactobacillus plantarum NCUF201.1(5%, 0.5%, 1.0%, and 1.5%). After TS was degraded efficiently, fermented TSC can be presumed a potential feed raw material.

尽管茶籽饼（TSC）具有很高的营养价值，但其茶皂素（TS）的高含量限制了其作为饲料的潜力。这项研究旨在通过皂苷降解菌株降解TSC中的TS，并使用多菌株发酵方法提高其营养价值和适口性。从油茶油茶土壤中分离出三种降解皂素的菌株，并将其鉴定为柠檬酸杆菌。FCTS301，Pantoea sp.。FCTS302和Enterobacter sp。FCTS303。单因素实验表明，柠檬酸杆菌属。FCTS301的TS降解率最高。柠檬酸杆菌的响应面分析sp。FCTS301的最佳培养条件为：初始pH值为7.2，培养温度为34.2°C，接种量为7.3％，搅拌速度为150 rpm，TS浓度为10.0 g / L。在这些条件下，最大降解率为82.6％。通过多菌株发酵实验获得了TSC的发酵过程。考虑到每组的蛋白质含量，粗纤维降解率和TS降解率，菌株的最佳接种量包括柠檬杆菌。FCTS301，米曲霉NCUF414，酿酒酵母NCUF306.5和植物乳杆菌NCUF201.1（5％，0.5％，1.0％和1.5％）。TS有效降解后，可以认为发酵的TSC是潜在的饲料原料。