Interleukin-33 (IL-33) is an IL-1 family cytokine with pleiotropic effects on diverse cell types. Dysregulated IL-33 signaling has been implicated in pregnancy-related disorders, including preeclampsia and recurrent pregnancy loss, and in ovarian function in women undergoing controlled ovarian stimulation for in vitro fertilization. To date, expression of IL-33 and its receptor subunit, ST2, in the female reproductive tract remains poorly characterized. We identify IL-33-expressing oocytes surrounded by ST2-expressing granulosa cells at all stages of follicular development, in addition to IL-33+ and ST2+ non-endothelial cells in the ovarian stroma and theca layer in ovaries from adult mice. These expression patterns are similar in estrus- and diestrus-stage adults and in pubescent mice, suggesting a role for IL-33 signaling in ovarian function throughout development and in the estrous cycle. In the uterus, we find expression of IL-33 and ST2 in glandular and luminal epithelia during estrus and at the initiation of pregnancy. Uterine IL-33 expression was modulated by the estrous cycle and was reduced in pubescent females. Last, superovulation increases transcripts for IL-33 and the soluble form of ST2 (sST2) in ovaries, and for IL-33 in uteri. Collectively, our findings lay the foundation for studies identifying cell type-specific requirements for IL-33/ST2 signaling in the establishment and maintenance of mouse pregnancy.

中文翻译：

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白细胞介素33和ST2在小鼠生殖道中的动态表达受到超排卵的影响。

白介素33（IL-33）是一种IL-1家族细胞因子，对多种细胞类型具有多效作用。IL-33信号转导失调与妊娠相关疾病有关，包括先兆子痫和复发性流产，以及在接受受控卵巢刺激进行体外受精的妇女中，卵巢功能异常。迄今为止，在雌性生殖道中IL-33及其受体亚基ST2的表达仍然很差。我们确定在成年小鼠卵巢基质和卵泡膜层中的IL-33 +和ST2 +非内皮细胞外，在卵泡发育的各个阶段都发现被表达ST2的颗粒细胞包围的表达IL-33的卵母细胞。这些表达模式在发情期和发情期成年期以​​及青春期小鼠中相似，提示IL-33信号在整个发育过程和动情周期中在卵巢功能中发挥作用。在子宫中，我们发现在发情期和怀孕初期，腺体和管腔上皮中有IL-33和ST2的表达。子宫IL-33的表达受发情周期的调节，在青春期女性中降低。最后，超排卵增加卵巢中IL-33和ST2（sST2）可溶性形式的转录本，子宫中IL-33的转录本增加。总的来说，我们的发现为确定小鼠妊娠建立和维持中IL-33 / ST2信号传导的细胞类型特异性要求的研究奠定了基础。子宫IL-33的表达受发情周期的调节，在青春期女性中降低。最后，超排卵增加卵巢中IL-33和ST2（sST2）可溶性形式的转录本，子宫中IL-33的转录本增加。总的来说，我们的发现为确定小鼠妊娠建立和维持中IL-33 / ST2信号传导的细胞类型特异性要求的研究奠定了基础。子宫IL-33的表达受发情周期的调节，在青春期女性中降低。最后，超排卵增加卵巢中IL-33和ST2（sST2）可溶性形式的转录本，子宫中IL-33的转录本增加。总的来说，我们的发现为确定小鼠妊娠建立和维持中IL-33 / ST2信号传导的细胞类型特异性要求的研究奠定了基础。