Apolipoprotein CIII (apoCIII) is associated with triglyceride (TG)-rich particles like VLDL and exerts an inhibitory effect of lipoprotein lipase. Increased levels are related to cardiovascular diseases and diabetes and therefore apoCIII has been proposed as a useful biomarker. Even if several commercial assays for measuring apoCIII in human plasma/serum are available, data is scarce concerning their reliability and none is used clinically. In the present study a comparative investigation has been done. Two ELISA-based methods (Cusabio Biotech and Assay Pro) and one nephelometric assay (Siemens Healthcare) were investigated. Serum and plasma samples were obtained from healthy volunteers and from samples sent to the Laboratory of Clinical Chemistry, preferably with higher levels of TGs. The Cusabio Biotech assay did not yield any valid results. However, both the methods from Assay Pro and Siemens Healthcare showed good performance with similar dynamic ranges. The latter assay had lower CV and required less work. In healthy individuals, apoCIII levels were not affected by fasting, freezing or thawing, nor did we find any gender differences. Individuals with elevated levels of TG displayed higher apoCIII values. Females with oral intake of contraceptives had higher levels. In conclusion, the nephelometric assay showed the best performance with the lowest CV, was less labor intensive than an assay based on ELISA and could therefore be suitable for clinical use.

中文翻译：

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人载脂蛋白CIII水平；三种测定方法的评价。

载脂蛋白CIII（apoCIII）与富含甘油三酸酯（TG）的颗粒（如VLDL）相关，并发挥了对脂蛋白脂肪酶的抑制作用。升高的水平与心血管疾病和糖尿病有关，因此已提出apoCIII作为有用的生物标志物。即使有几种用于测量人血浆/血清中apoCIII的商业化检测方法，也缺乏关于其可靠性的数据，临床上也没有使用。在本研究中，已经进行了比较研究。研究了两种基于ELISA的方法（Cusabio Biotech和Assay Pro）和一种浊度分析（Siemens Healthcare）。血清和血浆样品取自健康志愿者，并取自送往临床化学实验室的样品，最好含较高水平的TG。Cusabio Biotech分析未产生任何有效结果。但是，Assay Pro和Siemens Healthcare的方法在相似的动态范围内均显示出良好的性能。后一种测定法的CV较低，需要的工作量较少。在健康个体中，apoCIII水平不受空腹，冰冻或解冻的影响，我们也没有发现任何性别差异。TG水平升高的个体显示出更高的apoCIII值。口服避孕药的女性水平较高。总之，比浊法显示的最佳性能和最低的CV，比基于ELISA的测定的劳动强度小，因此可能适合临床使用。空腹，冰冻或解冻不会影响apoCIII的水平，我们也没有发现任何性别差异。TG水平升高的个体显示出更高的apoCIII值。口服避孕药的女性水平较高。总而言之，比浊法显示的最佳性能和最低的CV，比基于ELISA的测定的劳动强度低，因此可能适合临床使用。空腹，冰冻或解冻不会影响apoCIII的水平，我们也没有发现任何性别差异。TG水平升高的个体显示出更高的apoCIII值。口服避孕药的女性水平较高。总之，比浊法显示的最佳性能和最低的CV，比基于ELISA的测定的劳动强度低，因此可能适合临床使用。