This study investigated the relationship between acrosome reactions and fatty acid composition with respect to fertility in boar sperm. The acrosome reaction was induced more than 85% by 60 mM methyl-beta-cyclodextrin (MBCD), and plasma membrane integrity was significantly reduced dependent on the MBCD level in boar sperm (p < .05). The acrosome-reacted sperm exhibited significantly higher saturated fatty acids (SFAs) and lower polyunsaturated fatty acids (PUFAs) composition compared to the non-acrosome reaction group (p < .0001). In addition, the PUFAs, C22:5n-6 (docosapentaenoic acid [DPA]; p < .01) and C22:6n-3 (docosahexaenoic acid [DHA]; p < .0001) were significantly decreased, and cleavage and blastocyst formation of oocytes were significantly (p < .0001) decreased in acrosome-reacted sperm relative to non-acrosome-reacted sperm. Moreover, acrosome reaction was positively correlated with SFAs, whereas negatively correlated with PUFAs, of the PUFAs, the DPA (p = .0005) and DHA (p = <.0001) were negatively correlated with the acrosome reaction. Therefore, these results suggest that the PUFAs composition of sperm is closely involved in acrosome reaction in pigs.

中文翻译：

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公猪精子顶体反应与多不饱和脂肪酸组成之间的关系。

这项研究调查了顶体反应和脂肪酸组成与公猪精子繁殖力之间的关系。60 mM甲基-β-环糊精（MBCD）诱导顶体反应超过85％，并且取决于公猪精子中MBCD的水平，血浆膜的完整性显着降低（p <.05）。与非顶体反应组相比，顶体反应的精子表现出明显更高的饱和脂肪酸（SFA）和更低的多不饱和脂肪酸（PUFA）组成（p <.0001）。此外，PUFAs，C22：5n-6（二十二碳五烯酸[DPA]； p <.01）和C22：6n-3（二十二碳六烯酸[DHA]； p <.0001）显着减少，并且卵裂和囊胚形成的卵母细胞数显着（p <。0001）顶体反应的精子相对于非顶体反应的精子减少。此外，在PUFA中，顶体反应与SFA正相关，而与PUFA负相关，DPA（p = .0005）和DHA（p = .0001）与顶体反应呈负相关。因此，这些结果表明，精子的PUFA组成与猪的顶体反应密切相关。