Glyphosate is the main herbicide currently used in the world due to wide applicability and efficiency in controlling weeds in many crops. However, its overuse may lead to undesirable impacts on the environment and to human health in the long run. This present study aimed to optimize and validate solid phase extraction (SPE) using an anionic resin for the simultaneous and direct determination of glyphosate and aminomethylphosphonic acid (AMPA) in water samples using high-performance liquid chromatography combined with inductively coupled plasma with triple quadrupole mass spectrometer (HPLC-ICP-MS/MS). The results showed that recovery percentage and relative standard deviation were 103.9 ± 7.9 and 99.40 ± 9.9% for glyphosate and AMPA, respectively. The validation certified that the method was precise, accurate, linear, and selective, with a limit of quantification of 1.09 and 0.29 μg L⁻¹ for glyphosate and AMPA, respectively. The optimized methodology reached the concentration factor of 250 times and was successfully applied to analyze water samples from hydroponic cultivation of the eucalyptus seedlings. The results showed that the exudation process occurs at glyphosate doses starting from 2 L ha⁻¹.

草甘膦是目前世界上使用的主要除草剂，具有广泛的适用性和对多种作物杂草的有效控制作用。然而，从长远来看，过度使用可能会对环境和人类健康造成不良影响。本研究旨在优化和验证使用阴离子树脂的固相萃取 (SPE)，使用高效液相色谱结合三重四极杆质量的电感耦合等离子体同时直接测定水样中的草甘膦和氨甲基膦酸 (AMPA)光谱仪（HPLC-ICP-MS/MS）。结果显示，草甘膦和 AMPA 的回收率和相对标准偏差分别为 103.9 ± 7.9 和 99.40 ± 9.9%。验证表明该方法精密、准确、线性、选择性强，草甘膦和AMPA的定量限分别为1.09和0.29 μg L ^-1 。优化方法的浓缩倍数达到250倍，并成功应用于桉树苗水培水样的分析。结果表明，草甘膦剂量从2 L ha ^-1开始出现渗出过程。