OBJECTIVE To compare the effects of the complex triamcinolone acetonide-hydroxypropyl-β-cyclodextrin (TA-CD) on in vitro inflamed primary human articular chondrocytes in the presence or absence of the mixture hyaluronic acid-Chitlac, a lactose-modified chitosan (HA-CTL). DESIGN Changes in cell viability and pro-inflammatory cytokines gene expression were analyzed in human chondrocytes using an in vitro model of macrophage-mediated inflammation. Human monocytes U937 were differentiated to macrophages by phorbol 12-myristate 13-acetate (PMA) and lipopolysaccharides (LPS). The anti-inflammatory effects of the complex TA-CD and HA-CTL mixture were assessed on chondrocytes exposed for 24 hours to U937 conditioned medium (CM), by quantitative polymerase chain reaction analysis. RESULTS The TA-CD viability was enhanced by the presence of the HA-CTL mixture in chondrocyte cultures. The exposure of cells to CM significantly increased interleukin-1β and interleukin-6 gene expression, and when the complex TA-CD was added to the inflamed cells, gene transcription of cytokines was restored to near baseline values, both in the presence or in the absence of HA-CTL mixture. CONCLUSION The addition of HA-CTL mixture significantly attenuated cytotoxicity induced by TA and preserved the anti-inflammatory effects, thus confirming the chondroprotective role of the HA-CTL mixture.

中文翻译：

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透明质酸-Chitlac-曲西莫龙丙酮化物-β-环糊精复合物对人软骨细胞的活力和抗炎作用。

目的比较复合曲安奈德-羟丙基-β-环糊精 (TA-CD) 在存在或不存在混合透明质酸-Chitlac、乳糖改性壳聚糖 (HA- CTL）。设计 使用巨噬细胞介导炎症的体外模型分析人软骨细胞中细胞活力和促炎细胞因子基因表达的变化。人单核细胞 U937 通过佛波醇 12-肉豆蔻酸酯 13-乙酸 (PMA) 和脂多糖 (LPS) 分化为巨噬细胞。通过定量聚合酶链反应分析，对暴露于 U937 条件培养基 (CM) 24 小时的软骨细胞评估复合 TA-CD 和 HA-CTL 混合物的抗炎作用。结果HA-CTL混合物在软骨细胞培养物中的存在增强了TA-CD的活力。细胞暴露于 CM 显着增加了白细胞介素 1β 和白细胞介素 6 基因的表达，当将复合物 TA-CD 添加到发炎的细胞中时，细胞因子的基因转录恢复到接近基线值，无论是在存在还是在没有 HA-CTL 混合物。结论 HA-CTL混合物的加入显着减弱了TA诱导的细胞毒性并保留了抗炎作用，从而证实了HA-CTL混合物的软骨保护作用。在 HA-CTL 混合物存在或不存在的情况下，细胞因子的基因转录恢复到接近基线值。结论 HA-CTL混合物的加入显着减弱了TA诱导的细胞毒性并保留了抗炎作用，从而证实了HA-CTL混合物的软骨保护作用。在 HA-CTL 混合物存在或不存在的情况下，细胞因子的基因转录恢复到接近基线值。结论 HA-CTL混合物的加入显着减弱了TA诱导的细胞毒性并保留了抗炎作用，从而证实了HA-CTL混合物的软骨保护作用。