The mesoniviruses (MESOVs) belong to the newly described Mesoniviridae family (Order: Nidovirales). They have never been reported in Senegal until recently during a study in arbovirus emergence with the detection of a new species of MESOV named Dianke virus (DKV) from common mosquitoes from eastern Senegal. Actually, their vector competence for this newly described DKV is unknown. We, therefore, estimated the vector competence of Culex tritaeniorhynchus, Culex quinquefasciatus, Aedes aegypti, and Anopheles gambiae mosquitoes collected in Senegal for DKV using oral infection. Whole bodies, legs/wings, and saliva samples were tested for DKV by RT-PCR to estimate infection, dissemination, and transmission rates. The infectivity of virus particles in the saliva was confirmed by infecting C6/36 cells. Virus transmission rates were up to 95.45% in Culex tritaeniorhynchus, 28% in Cx. quinquefasciatus and 9.09% in Aedes aegypti. Viral particles in the saliva were confirmed infectious by C6/36 cell culture. An. gambiae was able to disseminate DKV only at 20 days post-infection. This study shows that Culex mosquitoes are more competent than Ae. aegypti for DKV, while Anopheles gambiae is not likely a competent vector.

中文翻译：

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塞内加尔嗜人蚊子对新的mesonivirus的媒介能力。

mesoniviruses（MESOVs）属于新描述的Mesoniviridae家族（订单：Nidovirales）。直到最近在塞内加尔才报道过这种虫媒病毒出现的研究，该研究是从塞内加尔东部的普通蚊子中检测出一种新的MESOV物种，称为“点科病毒”（DKV）。实际上，他们对这种新描述的DKV的媒介能力并不了解。因此，我们估计了塞内加尔收集的口疮感染的库蚊Tritaeniorhynchus，库蚊，昆蚊，埃及伊蚊和冈比亚按蚊的媒介能力。通过RT-PCR对全身，腿/翼和唾液样品进行DKV测试，以评估感染，传播和传播率。通过感染C6 / 36细胞来确认唾液中病毒颗粒的感染性。病毒传播率高达95。Culex tritaeniorhynchus中为45％，Cx中为28％。埃及伊蚊中有quinquefasciatus和9.09％。通过C6 / 36细胞培养证实唾液中的病毒颗粒具有传染性。一个。冈比亚仅在感染后20天才传播DKV。这项研究表明库蚊蚊比Ae更能干。aegypti用于DKV，而冈比亚按蚊（Anopheles gambiae）不太可能是有效的媒介。