1. In this study, classical and molecular microbiological methods for detection and quantification of Campylobacter spp. were used to estimate their prevalence in faecal samples and skin swabs collected from 31 broiler flocks (20 farms) in Portugal, and measure the impact of transport-related factors on the expected rising excretion rates from the farm to the slaughterhouse.

2. Data on husbandry practices and transport conditions were gathered, including time in transit, distance travelled or ante-mortem plant-holding time.

3. A generalised linear mixed model was used to evaluate the significance of a potential post-transport rise in Campylobacter spp. counts and to assess risk determinants.

4. At least one flock tested positive for Campylobacter spp. in 80% of the sampled farms. At the slaughterhouse, Campylobacter spp. were detected in all faecal samples, C. jejuni being the most commonly isolated.

5. A post-transport rise of Campylobacter spp. counts from skin swabs was observed using classical microbiological methods (from a mean of 1.43 to 2.40 log₁₀ CFU/cm²) and molecular techniques (from a mean of 2.64 to 3.31 log₁₀ genome copies/cm²).

6. None of the husbandry practices or transport-related factors were found to be associated with Campylobacter spp. counts.

7. This study highlights the need for more research to better understand the multi-factorial nature of Campylobacter spp., a public health threat that was found to be highly prevalent in a sample of Portuguese poultry farms.

1.在这项研究中，检测和定量弯曲杆菌属的经典和分子微生物学方法。分别从葡萄牙31个肉鸡群（20个农场）收集的粪便样本和皮肤拭子中评估它们的流行率，并测量运输相关因素对从农场到屠宰场预期排泄率上升的影响。

2.收集了有关饲养方式和运输条件的数据，包括运输时间，行进距离或宰前植物保留时间。

3.使用广义线性混合模型来评估弯曲杆菌属菌在运输后潜在升高的意义。计数并评估风险决定因素。

4.至少有一个鸡群弯曲杆菌属呈阳性。在80％的采样农场中。在屠宰场，弯曲杆菌属。在所有粪便样本中都检测到了空肠弯曲杆菌，最常见的是空肠弯曲杆菌。

5.运输后弯曲杆菌的上升。使用经典的微生物学方法（平均值为1.43至2.40 log ₁₀ CFU / cm ²）和分子技术（平均值为2.64至3.31 log ₁₀基因组拷贝/ cm ²）观察到了拭子计数。

6.没有发现与弯曲杆菌属有关的任何饲养方式或运输相关因素。计数。

7.这项研究强调需要更多的研究以更好地了解弯曲杆菌属的多因素性质，弯曲杆菌属是一种公共卫生威胁，在葡萄牙的一个家禽养殖场样本中普遍存在。