Background. Sodium valproate has anticonvulsant activity and is structurally different to conventional antiepileptic drugs. The problem with valproic acid is the lack of a chromophore, which means that gas chromatography is the sole assay methodology. The introduction of benzoyl and phenyl groups to the molecule is a useful derivatisation, which enables the creation of detectable chromophores for HPLC analysis for pharmaceutical dosages as well as biological systems. Methodology. Sodium valproate was derivatised by the addition of a chromophore to its structure by introducing a methyl benzoyl or a phenyl group. Trichlorophenol and 2-hydroxyacetophenone were used to introduce phenyl and benzoyl groups to valproic acid, respectively. The reaction used was estrification reaction using coupling agents. An analytical method was then developed and validated using reverse-phase HPLC. The method was validated for parameters like linearity, range, accuracy precision, and robustness. Results. The developed method was easy and feasible and can be applied to both routine analysis and bioanalysis. The method was very sensitive and could quantify valproic acid at a very low concentration of 0.75 × 10⁻⁵ mg/ml. The developed method was found to be linear (R² = 0.997), accurate, precise, and robust. Conclusion. The proposed chemical derivatisation and the developed analytical method are novel. The developed analytical procedure is the first of its kind; it is easy and feasible and can be used to quantify and detect sodium valproate at very low concentrations compared to other available methods in the literature.

中文翻译：

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通过化学衍生法开发丙戊酸钠的分析方法。

背景。丙戊酸钠具有抗惊厥活性，在结构上与常规抗癫痫药物不同。丙戊酸的问题是缺乏发色团，这意味着气相色谱法是唯一的测定方法。向分子中引入苯甲酰基和苯基是一种有用的衍生化方法，它能够产生可检测的发色团，用于药物剂量和生物系统的 HPLC 分析。方法. 丙戊酸钠是通过引入甲基苯甲酰基或苯基在其结构中添加发色团来衍生的。三氯苯酚和 2-羟基苯乙酮分别用于将苯基和苯甲酰基引入丙戊酸。使用的反应是使用偶联剂的酯化反应。然后使用反相 HPLC 开发和验证了一种分析方法。对该方法的线性度、范围、准确度、精密度和稳健性等参数进行了验证。结果。所开发的方法简单易行，可应用于常规分析和生物分析。^{该方法非常灵敏，可以在 0.75 × 10 -5}  mg/ml的极低浓度下定量丙戊酸。发现所开发的方法是线性的（R²  = 0.997)，准确、精确和稳健。结论。所提出的化学衍生化和开发的分析方法是新颖的。开发的分析程序是同类中的第一个；与文献中其他可用方法相比，该方法简单可行，可用于在极低浓度下定量和检测丙戊酸钠。