Microglia colonize the developing vertebrate central nervous system coincident with the detection of developmental apoptosis. Our understanding of apoptosis in intact tissue in relation to microglial clearance of dying cells is largely based on fixed samples, which is limiting given that microglia are highly motile and mobile phagocytes. Here, we used a system of microglial depletion and in vivo real‐time imaging in zebrafish to directly address microglial phagocytosis of apoptotic cells during normal retinal development, the relative timing of phagocytosis in relation to apoptotic progression, and the contribution of P2RY12 signaling to this process.

中文翻译：

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发育中的视网膜偶合吞噬作用中的小胶质细胞通过 P2RY12 信号传导进行凋亡。

小胶质细胞在发育中的脊椎动物中枢神经系统中定居，同时检测到发育性细胞凋亡。我们对完整组织中与死亡细胞的小胶质细胞清除相关的细胞凋亡的理解主要基于固定样本，鉴于小胶质细胞是高度运动和移动的吞噬细胞，因此这是有限的。在这里，我们在斑马鱼中使用小胶质细胞耗竭系统和体内实时成像来直接解决正常视网膜发育过程中小胶质细胞对凋亡细胞的吞噬作用、吞噬作用与凋亡进展的相对时间以及 P2RY12 信号传导对此的贡献过程。