The vanilloid-type transient receptor potential (TRPV) channel is reported to be the molecular target of the commercial insecticide pymetrozine, which specifically disrupts the feeding of plant sap-sucking insects. However, the functions of TRPV channels in plant sap-sucking insects have not been fully elucidated. In the present study, RNA interference was used to investigate the effects of the knockdown of TRPV genes (Nan and Iav) on the mortality, locomotion, and feeding behavior of an important plant-feeding insect pest in rice, the brown planthopper, Nilaparvata lugens. Injecting dsRNA of Nan and Iav into fourth-instar nymphs significantly knocked down the target genes. The injection of dsNan or dsIav did not affect any morphological phenotype (including leg extension) of N. lugens nymphs and adults. Knockdown of Nan or Iav resulted in significantly decreased climbing activity against top plants but did not influence the leg-griping strength of adults. Knockdown of Nan resulted in a significantly elevated mortality of N. lugens in the observation period of 7 d after injection, whereas no significant difference in survival rates 7 d after injection was found between dsIav-injected and dsGFP-injected insects. Electropenetrographic (EPG) recordings indicated that knockdown of Nan and Iav reduced the ingestion activity in the rice phloem tissues of N. lugens. Knockdown of Nan and Iav significantly reduced the amount of honeydew excreted by N. lugens. Our findings indicated a relationship between TRPV and N. lugens locomotion and feeding behavior, which may help to fully elucidate the functions of TRPV in insects.

中文翻译：

---

TRPV基因的敲除影响褐飞虱的运动和摄食行为。

据报道，类香草酸型瞬时受体电位（TRPV）通道是商业杀虫剂pymetrozine的分子靶标，后者专门破坏了植物吸液昆虫的摄食。然而，尚未完全阐明TRPV通道在植物吸液昆虫中的功能。在本研究中，RNA干扰被用于研究TRPV基因的敲除（Nan和Iav）对水稻，褐飞虱，Nilaparvata lugens中一种重要的以植物为食的害虫的死亡率，运动和摄食行为的影响。 。将Nan和Iav的dsRNA注入四龄若虫会显着击倒靶基因。注射dsNan或dsIav不会影响N. lugens若虫和成虫的任何形态学表型（包括腿伸）。击倒Nan或Iav导致针对顶级植物的攀登活动明显减少，但不影响成年人的握腿力。在注射后7 d的观察期内，敲除Nan会导致发光猪笼草的死亡率显着提高，而注射dsIav的昆虫和注射dsGFP的昆虫在注射后7 d的存活率没有显着差异。电镜（EPG）记录表明，敲除Nan和Iav会降低黄褐猪笼草在水稻韧皮部的摄食活性。击倒Nan和Iav大大减少了N. lugens分泌的蜜露数量。我们的研究结果表明TRPV和猪笼草的运动与摄食行为之间存在关系，这可能有助于充分阐明TRPV在昆虫中的功能。