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Impediment to growth and yeast-to-hyphae transition in Candida albicans by copper oxide nanoparticles
Biofouling ( IF 2.6 ) Pub Date : 2020-01-02 , DOI: 10.1080/08927014.2020.1715371
Alwar Ramanujam Padmavathi 1 , Sriyutha Murthy P 1, 2 , Arindam Das 3, 4 , Arumugam Priya 5 , T J Sushmitha 5 , Shunmugiah Karutha Pandian 5 , Subba Rao Toleti 1, 2
Affiliation  

Abstract The effects of two prominent copper oxide nanoparticles (CuO-NP and Cu2O-NP), with the oxidation state of Cu++ (cupric) and Cu+ (cuprous), on Candida albicans were evaluated. CuO-NP and Cu2O-NP were synthesized and characterized by XRD, FESEM, HR-TEM and Zeta potential. At sub-MIC (50 µg ml−1), both cupric and cuprous oxide NPs prevented yeast-to-hyphae switching and wrinkling behaviour in C. albicans. The mechanism for the antifungal action of the two NPs differed; CuO-NP significantly elicited reactive oxygen species, whereas membrane damage was more pronounced with Cu2O-NP. Real time PCR analysis revealed that CuO-NP suppressed the morphological switching of yeast-to-hyphae by down-regulating cph1, hst7 and ras1 and by up-regulation of the negative regulator tup1. In comparison, Cu2O-NP resulted in down-regulation of ras1 and up-regulation of the negative regulators nrg1 and tup1. Between the two NPs, CuO exhibited increased antifungal activity due to its stable oxidation state (Cu++) and its smaller dimensions compared with Cu2O-NP. Graphical Abstract

中文翻译:

氧化铜纳米颗粒对白色念珠菌生长和酵母菌向菌丝转变的阻碍

摘要 评估了两种主要氧化铜纳米粒子(CuO-NP 和 Cu2O-NP)对白色念珠菌的影响,其氧化态为 Cu++(铜)和 Cu+(亚铜)。合成了CuO-NP和Cu2O-NP,并通过XRD、FESEM、HR-TEM和Zeta电位对其进行了表征。在亚 MIC (50 µg ml-1) 下,铜和氧化亚铜 NPs 都阻止了酵母菌到菌丝的转换和白色念珠菌的起皱行为。两种纳米颗粒的抗真菌作用机制不同;CuO-NP 显着引发活性氧,而 Cu2O-NP 对膜的损伤更明显。实时 PCR 分析显示,CuO-NP 通过下调 cph1、hst7 和 ras1 以及上调负调节因子 tup1 来抑制酵母菌向菌丝的形态转换。相比下,Cu2O-NP 导致 ras1 的下调和负调节因子 nrg1 和 tup1 的上调。在两种纳米颗粒之间,与 Cu2O-NP 相比,CuO 由于其稳定的氧化态 (Cu++) 和更小的尺寸而表现出更高的抗真菌活性。图形概要
更新日期:2020-01-02
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