Coenzyme Q10 (CoQ10)-an essential cofactor in the respiratory electron transport chain-has important pharmaceutical and healthcare applications. Farnesol (FOH)-an acyclic sesquiterpene alcohol-has garnered interest owing to its valuable clinical and medical benefits. Here, the coproduction of CoQ10 and FOH in Rhodobacter sphaeroides GY-2 was greatly improved through the enhancement of intracellular NADPH availability. Transcription of pgi, gdhA, and nuocd was, respectively, inhibited using RNA interference to reduce intracellular NAD(P)H consumption. Moreover, zwf, gnd, and zwf + gnd were overexpressed to enhance the pentose phosphate pathway, resulting in improved NADPH availability in most metabolically engineered R. sphaeroides strains. RSg-pgi with RNAi of pgi combined with overexpression of gnd produced 55.05 mg/L FOH that is twofold higher than the parental strain GY-2, and 185.5 mg/L CoQ10 can be coproduced at the same time. In conclusion, improved carbon flux can be redirected toward NADPH-dependent biosynthesis through the enhancement of NADPH availability.

中文翻译：

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从球形红球菌共生产辅酶Q10和法尼醇的NADPH可用性增强。

辅酶Q10（CoQ10）-呼吸电子传输链中的重要辅因子-具有重要的药物和医疗保健应用。法尼索尔（FOH）-一种无环倍半萜烯醇-由于其宝贵的临床和医学益处而引起了人们的兴趣。在这里，球形红球菌GY-2中辅酶Q10和FOH的共同产生通过细胞内NADPH可用性的提高而大大提高。pgi，gdhA和nuocd的转录分别被RNA干扰抑制以减少细胞内NAD（P）H的消耗。此外，zwf，gnd和zwf + gnd被过表达以增强戊糖磷酸途径，从而在大多数经代谢工程改造的球形拟南芥菌株中提高了NADPH的利用率。带有pgi RNAi和gnd过度表达的RSg-pgi产生了55。可同时生产比亲本菌株GY-2高两倍的05 mg / L FOH和185.5 mg / L CoQ10。总之，通过提高NADPH的利用率，可以将改进的碳通量重新定向到NADPH依赖的生物合成。