It is of great economic interest to produce succinate from low-grade carbon sources, e.g., lignocellulosic biomass hydrolysate, which mainly contains glucose and xylose. Inactivation of the glucose uptake system PtsG was evaluated for succinate production from xylose-rich feedstocks. Strains with integration of succinate production modules into the chromosome of Escherichia coli were then constructed. These strains have better succinate production performance from xylose-rich feedstocks than strain FZ560 harboring pHL413KF1. Glucose utilization was enhanced in FZ661T by manipulation of the gal operon to allow efficient use of the high-concentration glucose in woody biomass hydrolysate. Up to 906.7 mM (107.0 g/L) succinate was produced from mixed sugars in fed-batch fermentation and more than 461.7 mM (54.5 g/L) succinate was produced from woody hydrolysate in a batch fermentation. In this study, FZ661T was able to produce succinate from woody hydrolysate in minimal medium efficiently, making it attractive for industrial applications in succinate production.

中文翻译：

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大肠杆菌的代谢工程，可在厌氧条件下由木质水解产物生产琥珀酸酯。

从低级碳源，例如主要包含葡萄糖和木糖的木质纤维素生物质水解产物生产琥珀酸酯具有重大的经济意义。评估葡萄糖摄取系统的失活，以从富含木糖的原料中产生琥珀酸盐。然后构建将琥珀酸生产模块整合到大肠杆菌染色体中的菌株。与富含pHL413KF1的菌株FZ560相比，这些菌株从富含木糖的原料中具有更好的琥珀酸盐生产性能。通过操纵gal操纵子可以提高FZ661T的葡萄糖利用率，从而有效利用木质生物质水解产物中的高浓度葡萄糖。在分批补料发酵中，混合糖可产生高达906.7 mM（107.0 g / L）的琥珀酸酯，超过461.7 mM（54。在分批发酵中由木质水解物产生5 g / L琥珀酸酯。在这项研究中，FZ661T能够在最少的培养基中有效地从木质水解产物生产琥珀酸酯，使其在琥珀酸酯生产的工业应用中具有吸引力。