Abstract Staphylococcus lugdunensis is an emerging high-virulent pathogen causative of hospital-acquired infections. Biofilm formation is a complex pathogenic process that leads to well-established bacterial communities. There is a paucity of data on the composition of the biofilm matrix among S. lugdunensis strains. Here, twenty-two S. lugdunensis clinical isolates, mainly from orthopaedic infections but also from other clinical sources, were sub-grouped by ribotyping and dendrogram analysis. Biofilms were analysed by fluorimetric methods based on FITC-Wheat Germ Agglutinin, SYPRO Ruby and TOTO-1 dyes to detect exopolysaccharides, proteins and extracellular DNA (eDNA), respectively. Biofilm morphology was investigated under confocal laser scanning microscopy (CLSM). Isolates displayed intriguing diversities in biofilm mass and matrix composition. The content of exopolysaccharides was found to be to be strongly associated with the biofilm mass (R2 = 0.882), while the content of proteins turned out to be weakly (R2 = 0.465) and that of eDNA very weakly associated (R2 = 0.202) to the biofilm mass.

中文翻译：

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新兴病原体路敦葡萄球菌的各种生物膜基质：胞外多糖、蛋白质、eDNA 及其与生物膜质量的相关性

摘要 路敦葡萄球菌是一种新出现的引起医院获得性感染的高毒力病原体。生物膜形成是一个复杂的致病过程，会导致建立完善的细菌群落。关于 S. lugdunensis 菌株中生物膜基质组成的数据很少。在这里，主要来自骨科感染但也来自其他临床来源的 22 种 S. lugdunensis 临床分离株通过核糖体分型和树状图分析进行了分组。通过基于 FITC-小麦胚芽凝集素、SYPRO Ruby 和 TOTO-1 染料的荧光法分析生物膜，分别检测胞外多糖、蛋白质和细胞外 DNA (eDNA)。在共聚焦激光扫描显微镜 (CLSM) 下研究生物膜形态。分离物在生物膜质量和基质组成方面显示出有趣的多样性。