The goals of this study were to increase the production of antroquinonol (AQ) and to elucidate the response mechanism of the cell membrane during the in situ extractive fermentation (ISEF) of Antrodia camphorata S-29. Through ISEF, the concentration of AQ reached a maximum of 146.1 ± 2.8 mg/L, which was approximately (7.4 ± 0.1)-fold that of the control (coenzyme Q0-induced fermentation). Transcriptome sequencing showed that four genes (FAD2, fabG, SCD, and FAS1) related to fatty acid biosynthesis were upregulated. FAD2 and SCD may regulate the increase in oleic acid (C18:1) and linoleic acid (C18:2) in the cell membrane of A. camphorata S-29, resulting in an increase in cell membrane permeability. AQ was successfully transferred to the n-tetradecane phase through the cell membrane, reducing product feedback inhibition and improving the production of AQ from A. camphorata S-29.

中文翻译：

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原位提取发酵中细胞膜通透性的增加改善了樟脑樟（Antrodia camphorata）S-29的蒽醌醇产量。

这项研究的目的是增加樟脑樟原S-29的原位提取发酵（ISEF）过程中，对苯二酚（AQ）的产生并阐明细胞膜的响应机制。通过ISEF，AQ的浓度最高达到146.1±2.8 mg / L，约为对照（辅酶Q0诱导的发酵）的（7.4±0.1）倍。转录组测序显示与脂肪酸生物合成相关的四个基因（FAD2，fabG，SCD和FAS1）被上调。FAD2和SCD可能调节樟脑草S-29细胞膜中油酸（C18：1）和亚油酸（C18：2）的增加，从而导致细胞膜通透性增加。AQ已通过细胞膜成功转移至正十四烷相，