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Zinc can counteract selection for ciprofloxacin resistance.
FEMS Microbiology Letters ( IF 2.2 ) Pub Date : 2020-02-27 , DOI: 10.1093/femsle/fnaa038
Michiel Vos 1 , Louise Sibleyras 1, 2 , Lai Ka Lo 1, 3, 4 , Elze Hesse 4, 5 , William Gaze 1 , Uli Klümper 1, 4, 5
Affiliation  

Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-characterized, experimental studies exploring their combined effects on AMR evolution are rare. It has previously been demonstrated that fluoroquinolone antibiotics such as ciprofloxacin can chelate metal ions. To investigate how ciprofloxacin resistance is affected by the presence of metals, we quantified selection dynamics between a ciprofloxacin-susceptible and a ciprofloxacin-resistant Escherichia coli strain across a gradient of ciprofloxacin concentrations in presence and absence of zinc. The presence of zinc reduced growth of both strains, while ciprofloxacin inhibited exclusively the susceptible one. When present in combination zinc retained its inhibitory effect, while ciprofloxacin inhibition of the susceptible strain was reduced. Consequently, the minimal selective concentration for ciprofloxacin resistance increased up to five-fold in the presence of zinc. Environmental pollution usually comprises complex mixtures of antimicrobial agents. In addition to the usual focus on additive or synergistic interactions in complex selective mixtures, our findings highlight the importance of antagonistic selective interactions when considering resistance evolution.

中文翻译:

锌可以抵消环丙沙星耐药性的选择。

抗菌素耐药性 (AMR) 已成为对公共卫生的最紧迫威胁之一。AMR 进化发生在临床和环境中,抗生素和重金属可以选择和共同选择 AMR。虽然抗生素和金属的选择性潜力越来越明显,但探索它们对 AMR 进化的综合影响的实验研究却很少。先前已证明氟喹诺酮类抗生素如环丙沙星可以螯合金属离子。为了研究环丙沙星耐药性如何受到金属存在的影响,我们量化了在存在和不存在锌的情况下,环丙沙星敏感和环丙沙星耐药大肠杆菌菌株之间的选择动态。锌的存在降低了两种菌株的生长,而环丙沙星仅抑制易感菌株。当组合存在时,锌保持其抑制作用,而环丙沙星对敏感菌株的抑制作用降低。因此,在锌存在的情况下,环丙沙星耐药性的最小选择浓度增加了五倍。环境污染通常包括抗菌剂的复杂混合物。除了通常关注复杂选择性混合物中的加性或协同相互作用外,我们的研究结果还强调了在考虑抗性演化时拮抗选择性相互作用的重要性。而环丙沙星对易感菌株的抑制作用降低。因此,在锌存在的情况下,环丙沙星耐药性的最小选择浓度增加了五倍。环境污染通常包括抗菌剂的复杂混合物。除了通常关注复杂选择性混合物中的加性或协同相互作用外,我们的研究结果还强调了在考虑抗性演化时拮抗选择性相互作用的重要性。而环丙沙星对易感菌株的抑制作用降低。因此,在锌存在的情况下,环丙沙星耐药性的最小选择浓度增加了五倍。环境污染通常包括抗菌剂的复杂混合物。除了通常关注复杂选择性混合物中的加性或协同相互作用外,我们的研究结果还强调了在考虑抗性演化时拮抗选择性相互作用的重要性。
更新日期:2020-04-17
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