Regenerative medicine literature has proposed mesenchymal stem/progenitor cell- (MSC-) mediated therapeutic approaches for their great potential in managing various diseases and tissue defects. Dental MSCs represent promising alternatives to nondental MSCs, owing to their ease of harvesting with minimally invasive procedures. Their mechanism of action has been attributed to their cell-to-cell contacts as well as to the paracrine effect of their secreted factors, namely, secretome. In this context, dental MSC-derived secretome/conditioned medium could represent a unique cell-free regenerative and therapeutic approach, with fascinating advantages over parent cells. This article reviews the application of different populations of dental MSC secretome/conditioned medium in in vitro and in vivo animal models, highlights their significant implementation in treating different tissue’ diseases, and clarifies the significant bioactive molecules involved in their regenerative potential. The analysis of these recent studies clearly indicate that dental MSCs’ secretome/conditioned medium could be effective in treating neural injuries, for dental tissue regeneration, in repairing bone defects, and in managing cardiovascular diseases, diabetes mellitus, hepatic regeneration, and skin injuries, through regulating anti-inflammatory, antiapoptotic, angiogenic, osteogenic, and neurogenic mediators.

中文翻译：

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牙干细胞衍生的分泌组/条件培养基：再生治疗应用的未来。


再生医学文献提出了间充质干细胞/祖细胞（MSC）介导的治疗方法，因为它们在治疗各种疾病和组织缺陷方面具有巨大的潜力。牙科 MSC 是非牙科 MSC 的有前途的替代品，因为它们易于通过微创手术采集。它们的作用机制归因于它们的细胞间接触以及它们的分泌因子（即分泌组）的旁分泌作用。在这种情况下，牙科 MSC 衍生的分泌组/条件培养基可以代表一种独特的无细胞再生和治疗方法，与亲本细胞相比具有令人着迷的优势。本文综述了不同群体的牙科MSC分泌组/条件培养基在体外和体内动物模型中的应用，强调了它们在治疗不同组织疾病中的重要应用，并阐明了参与其再生潜力的重要生物活性分子。这些最近研究的分析清楚地表明，牙科间充质干细胞的分泌组/条件培养基可有效治疗神经损伤、牙组织再生、修复骨缺损以及治疗心血管疾病、糖尿病、肝再生和皮肤损伤。通过调节抗炎、抗凋亡、血管生成、成骨和神经生成介质。