Nowadays, there is an increasing need for sensitive real-time measurements of various analytes and monitoring of industrial products and environmental processes. Herein, we describe a fluorescence spectrometer in continuous flow mode in which the sample is fed to the flow cell using a peristaltic pump. The excitation beam is introduced to the sample chamber by an optical fiber. The fluorescence emitted upon excitation is collected at the right angle using another optical fiber and then transmitted to the fluorescence spectrometer which utilizes an array detector. The array detection, as a key factor in process analytical chemistry, made the fluorescence spectrometer suited for multiwavelength detection of the fluorescence spectrum of the analytes. After optimization of the experimental parameters, the system has been successfully employed for sensitive determination of four fluoroquinolone antibiotics such as ciprofloxacin, ofloxacin, levofloxacin, and moxifloxacin. The linear dynamic ranges of four fluoroquinolones were between 0.25 and 20 μg·mL⁻¹, and the detection limit of the method for ciprofloxacin, ofloxacin, levofloxacin, and moxifloxacin were 81, 36, 35, and 93 ng·mL⁻¹, respectively. Finally, the proposed system is carried out for determination of fluoroquinolones in some pharmaceutical formulations.

中文翻译：

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具有阵列检测功能的光纤耦合分光荧光计作为过程分析化学工具，用于氟喹诺酮类抗生素的连续流量监测。


如今，对各种分析物的灵敏实时测量以及工业产品和环境过程监测的需求日益增长。在这里，我们描述了一种连续流动模式的荧光光谱仪，其中使用蠕动泵将样品送入流动池。激发光束通过光纤引入样品室。使用另一根光纤以直角收集激发时发射的荧光，然后传输到使用阵列检测器的荧光光谱仪。阵列检测作为过程分析化学的关键因素，使得荧光光谱仪适用于分析物荧光光谱的多波长检测。经过实验参数优化，该系统已成功用于环丙沙星、氧氟沙星、左氧氟沙星、莫西沙星等4种氟喹诺酮类抗生素的灵敏测定。 4种氟喹诺酮类药物的线性动态范围在0.25~20 μg ·mL ^-1之间，环丙沙星、氧氟沙星、左氧氟沙星和莫西沙星的方法检出限分别为81、36、35和93 ng·mL ^-1 。分别。最后，所提出的系统用于测定一些药物制剂中的氟喹诺酮类药物。