Objective: The folate-modified graphene oxide (GO-FA), which had good stability and biocompatibility on rat glioma cells was successfully prepared.

Methods: The formation and composition of GO-FA were confirmed by Scanning Electron Microscope (SEM), Transmission Electron Microscope (TEM), Fourier Transform Infrared Spectrum (FT-IR), Raman spectra and X-ray Photoelectron Spectroscopy (XPS spectra). The cell experiment suggested good biocompatibility of GO-FA on rat glioma cells.

Results: The experiment of GO-FA loading with Temozolomide (TMZ) showed that the maximum drug loading of GO-FA was 8.05 ± 0.20 mg/mg, with the drug loading rate of 89.52 ± 0.19 %. When TMZ was released from the folate-modified graphene oxide loading with temozolomide (GO-FATMZ), its release behavior in vitro showed strong pH dependence and sustained release property. The growth of rat glioma cells can be effectively inhibited by GO-FA-TMZ, with the cell inhibition rate as high as 91.72 ± 0.13 % at the concentration of 600 μg/mL and time of 72 h.

Conclusion: According to the above experimental results, this composite carrier has potential applications in drug delivery and cancer therapy.

目的：成功制备出对大鼠神经胶质瘤细胞具有良好稳定性和生物相容性的叶酸修饰氧化石墨烯（GO-FA）。

方法：通过扫描电子显微镜（SEM），透射电子显微镜（TEM），傅立叶变换红外光谱（FT-IR），拉曼光谱和X射线光电子能谱（XPS光谱）确定GO-FA的形成和组成。细胞实验表明GO-FA对大鼠神经胶质瘤细胞具有良好的生物相容性。

结果：用替莫唑胺（TMZ）进行GO-FA负载实验表明，GO-FA的最大负载量为8.05±0.20 mg / mg，负载率为89.52±0.19％。当TMZ从具有替莫唑胺（GO-FATMZ）的叶酸修饰的氧化石墨烯中释放时，其体外释放行为显示出较强的pH依赖性和持续释放性能。GO-FA-TMZ可有效抑制大鼠神经胶质瘤细胞的生长，在浓度为600μg/ mL，时间为72 h的条件下，细胞抑制率高达91.72±0.13％。

结论：根据以上实验结果，该复合载体在药物输送和癌症治疗中具有潜在的应用前景。