BACKGROUND Differentiation of lymphocytic-plasmacytic enteropathy (LPE) from small cell lymphoma (SCL) in cats can be challenging. HYPOTHESIS/OBJECTIVE Histology-guided mass spectrometry (HGMS) is a suitable method for the differentiation of LPE from SCL in cats. ANIMALS Forty-one cats with LPE and 52 cats with SCL. METHODS This is a retrospective clinicopathologic study. Duodenal tissue samples of 17 cats with LPE and 22 cats with SCL were subjected to HGMS, and the acquired data were used to develop a linear discriminate analysis (LDA) machine learning algorithm. The algorithm was subsequently validated using a separate set of 24 cats with LPE and 30 cats with SCL. Cases were classified as LPE or SCL based on a consensus by an expert panel consisting of 5-7 board-certified veterinary specialists. Histopathology, immunohistochemistry, and clonality testing were available for all cats. The panel consensus classification served as a reference for the calculation of test performance parameters. RESULTS Relative sensitivity, specificity, and accuracy of HGMS were 86.7% (95% confidence interval [CI]: 74.5%-98.8%), 91.7% (95% CI: 80.6%-100%), and 88.9% (95% CI: 80.5%-97.3%), respectively. Comparatively, the clonality testing had a sensitivity, specificity, and accuracy of 85.7% (95% CI: 72.8%-98.7%), 33.3% (95% CI: 14.5%-52.2%), and 61.5% (95% CI: 48.3%-74.8%) relative to the panel decision. CONCLUSIONS AND CLINICAL IMPORTANCE Histology-guided mass spectrometry was a reliable technique for the differentiation of LPE from SCL in duodenal formalin-fixed paraffin-embedded samples of cats and might have advantages over tests currently considered state of the art.

中文翻译：

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使用组织学指导的质谱法鉴别猫的淋巴细胞-浆细胞性肠病和小细胞淋巴瘤。

背景技术猫的淋巴细胞性浆细胞性肠病（LPE）与小细胞淋巴瘤（SCL）的区别可能具有挑战性。假设/目标组织学指导质谱法（HGMS）是区分猫中SPE和LPE的合适方法。动物有LPE的猫41只，带SCL的猫52只。方法这是一项回顾性临床病理研究。对17只LPE猫和22只SCL猫的十二指肠组织样本进行HGMS处理，并将获得的数据用于开发线性判别分析（LDA）机器学习算法。随后使用包含24只LPE猫和30只SCL猫分别验证了该算法。根据由5-7名董事会认证的兽医专家组成的专家小组的共识，将病例分类为LPE或SCL。组织病理学，免疫组化，和克隆性测试适用于所有猫。小组共识分类可作为计算测试性能参数的参考。结果HGMS的相对敏感性，特异性和准确性分别为86.7％（95％置信区间[CI]：74.5％-98.8％），91.7％（95％CI：80.6％-100％）和88.9％（95％CI） ：80.5％-97.3％）。相比之下，克隆性测试的敏感性，特异性和准确性分别为85.7％（95％CI：72.8％-98.7％），33.3％（95％CI：14.5％-52.2％）和61.5％（95％CI： 48.3％-74.8％）。结论和临床意义组织学指导的质谱法是区分十二指肠福尔马林固定石蜡包埋的猫样本中LPE与SCL的可靠技术，它可能比目前认为最先进的测试具有优势。