当前位置: X-MOL 学术Cartilage › 论文详情
Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)
Influence of Glucose Concentration on Colony-Forming Efficiency and Biological Performance of Primary Human Tissue-Derived Progenitor Cells.
CARTILAGE ( IF 2.7 ) Pub Date : 2020-02-26 , DOI: 10.1177/1947603520906605
Venkata P Mantripragada 1 , Ryan Kaplevatsky 2 , Wes A Bova 1 , Cynthia Boehm 1 , Nancy A Obuchowski 3 , Ronald J Midura 1 , George F Muschler 1, 4
Affiliation  

OBJECTIVE Glucose concentrations used in current cell culture methods are a significant departure from physiological glucose levels. The study focuses on comparing the effects of glucose concentrations on primary human progenitors (connective tissue progenitors [CTPs]) used for cartilage repair. DESIGN Cartilage- (Outerbridge grade 1, 2, 3; superficial and deep zone cartilage), infrapatellar fatpad-, synovium-, and periosteum-derived cells were obtained from 63 patients undergoing total knee arthroplasty and cultured simultaneously in fresh chondrogenic media containing 25 mM glucose (HGL) or 5 mM glucose (NGL) for pairwise comparison. Automated ASTM-based quantitative image analysis was used to determine colony-forming efficiency (CFE), effective proliferation rates (EPR), and sulfated-proteoglycan (GAG-ECM) staining of the CTPs across tissue sources. RESULTS HGL resulted in increased cell cultures with CFE = 0 compared with NGL in all tissue sources (P = 0.049). The CFE in NGL was higher than HGL for superficial cartilage (P < 0.001), and contrary for synovium-derived CTPs (P = 0.046) when CFE > 0. EPR of the CTPs did not differ between the media in the 6-day assay time period (P = 0.082). The GAG-ECM area of the CTPs and their progeny was increased in presence of HGL (P = 0.027). CONCLUSION Glucose concentration is critical to progenitor's physiology and should be taken into account in the setting of protocols for clinical or in vitro cell expansion strategies.

中文翻译:

葡萄糖浓度对人原代组织衍生祖细胞集落形成效率和生物学性能的影响。

目标当前细胞培养方法中使用的葡萄糖浓度与生理葡萄糖水平有很大的不同。该研究的重点是比较葡萄糖浓度对用于软骨修复的原代人类祖细胞(结缔组织祖细胞 [CTP])的影响。设计 软骨-(Outerbridge 1、2、3 级;浅层和深层软骨)、髌下脂肪垫、滑膜和骨膜来源的细胞取自 63 名接受全膝关节置换术的患者,并在含有 25 mM 的新鲜软骨形成培养基中同时培养葡萄糖 (HGL) 或 5 mM 葡萄糖 (NGL) 用于成对比较。基于 ASTM 的自动定量图像分析用于确定集落形成效率 (CFE)、有效增殖率 (EPR)、和跨组织来源的 CTP 的硫酸化蛋白聚糖 (GAG-ECM) 染色。结果 在所有组织来源中,与 NGL 相比,HGL 导致 CFE = 0 的细胞培养增加(P = 0.049)。NGL 中的 CFE 高于浅表软骨的 HGL (P < 0.001),而当 CFE > 0 时,滑膜衍生的 CTP 则相反 (P = 0.046)。在 6 天测定中,CTP 的 EPR 在不同介质之间没有差异时间段(P = 0.082)。CTP 及其后代的 GAG-ECM 区域在 HGL 存在下增加(P = 0.027)。结论 葡萄糖浓度对祖细胞的生理机能至关重要,在制定临床或体外细胞扩增策略方案时应予以考虑。NGL 中的 CFE 高于浅表软骨的 HGL (P < 0.001),而当 CFE > 0 时,滑膜衍生的 CTP 则相反 (P = 0.046)。CTP 的 EPR 在 6 天测定中在培养基之间没有差异时间段(P = 0.082)。CTP 及其后代的 GAG-ECM 区域在 HGL 存在下增加(P = 0.027)。结论 葡萄糖浓度对祖细胞的生理机能至关重要,在制定临床或体外细胞扩增策略方案时应予以考虑。NGL 中的 CFE 高于浅表软骨的 HGL (P < 0.001),而当 CFE > 0 时,滑膜衍生的 CTP 则相反 (P = 0.046)。CTP 的 EPR 在 6 天测定中在培养基之间没有差异时间段(P = 0.082)。CTP 及其后代的 GAG-ECM 区域在 HGL 存在下增加(P = 0.027)。结论 葡萄糖浓度对祖细胞的生理机能至关重要,在制定临床或体外细胞扩增策略方案时应予以考虑。
更新日期:2020-04-20
down
wechat
bug