A high-performance liquid chromatography method employing a diode-array detector and mass spectrometry detector was developed, validated and implemented for determining Synephrine, Caffeine, Clenbuterol, Nandrolone, Testosterone and Methylhexaneamine in Nutritional supplements. The use of Nutritional supplements is widespread. Hazards relating to concentration, composition, individual contaminants, supplements interactions as well as positive doping results among athletes present increasing concerns regarding nutritional supplement consuming. The proposed method was validated according to the International Conference on the Harmonization of the Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) standards. The proposed method observed to be accurate, linear, precise, sensitive, required minimal sample preparation and uncomplicated mobile phase. The implementation of the proposed method on nine commercial supplements shows that inaccurate labeling for some supplements regarding the concentration of the ingredients.

中文翻译：

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HPLC-DAD和LC-MS测定营养补品中常见的合成代谢类固醇和兴奋剂。

开发，验证和实施了采用二极管阵列检测器和质谱检测器的高效液相色谱法，用于测定营养补品中的辛弗林，咖啡因，盐酸克伦特罗，南德隆，睾丸激素和甲基己胺。营养补品的使用非常普遍。与浓度，成分，单个污染物，补充剂相互作用以及运动员中积极的兴奋剂结果有关的危害引起了人们对营养补充剂消耗的日益关注。所提议的方法已根据国际统一人类用药品注册技术要求（ICH）标准的会议进行了验证。所提出的方法观察到准确，线性，精确，灵敏，需要最少的样品制备和简单的流动相。建议的方法在九种商业补品上的实施表明，某些补品关于成分浓度的标签不准确。