Oxytropis falcata Bunge, known as "the King of Herbs" in Tibetan medicine, is used for treatment of hyperpyrexia, pain, wounds, inflammation and anthrax. However, it is difficult to isolate compound with high-purity from O. falcata because of its complexity. In this work, an efficient method was successfully established for the separation of 3-hydroxy-3-methylglutaryl (HMG) flavonoid glycosides from O. falcata by 2D preparative chromatography (2D-HPLC). An ODS C18 preparative column was used for the first-dimension preparation, and the XCharge C18 preparative column with different separation selectivity to the ODS C18 stationary phase was used for the second-dimension preparation. Four HMG flavonoid glycosides (oxytroflavosides A-D) and two flavonoid glycosides (oxytroflavosides F and G) were separated with purities over 98%. Their structures were elucidated by nuclear magnetic resonance spectroscopy. The 2D-HPLC method used in this study is effective for preparative separation of HMG flavonoid glycosides from O. falcata. Additionally, this method shows great potential for the separation of flavonoid glycosides from other plant extracts.

中文翻译：

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使用2D制备色谱法从富氧棘豆中分离出3-羟基-3-甲基戊二烯基黄酮苷。

在藏医界被称为“草药之王”的富氧棘豆被用于治疗高热，疼痛，伤口，发炎和炭疽。但是，由于其复杂性，难以从镰刀菌中分离出高纯度的化合物。在这项工作中，成功地建立了一种有效的方法，可通过2D制备色谱法（2D-HPLC）从O. falcata分离3-羟基-3-甲基戊二烯基（HMG）类黄酮苷。第一维制备使用ODS C18制备柱，第二维制备使用对ODS C18固定相具有不同分离选择性的XCharge C18制备柱。分离出四个HMG类黄酮苷（氧合黄酮苷AD）和两个类黄酮苷（氧合黄酮苷F和G），纯度超过98％。通过核磁共振波谱阐明了它们的结构。本研究中使用的2D-HPLC方法可有效地从O. falcata分离制备HMG类黄酮苷。另外，该方法显示出从其他植物提取物中分离出黄酮苷的巨大潜力。