In addition to mesenchymal stem cells, adipose-derived stem/stromal cells (ASCs) are an attractive source for a large variety of cell-based therapies. One of their most important potential applications is related to the regeneration of bone tissue thanks to their capacity to differentiate in bone cells. However, this requires a proper control of their osteogenic differentiation, which depends not only on the initial characteristics of harvested cells but also on the conditions used for their culture. In this review, we first briefly describe the preclinical and clinical trials using ASCs for bone regeneration and present the quantitative parameters used to characterize the osteogenic differentiation of ASCs. We then focus on the soluble factors influencing the osteogenic differentiation of ACS, including the steroid hormones and various growth factors, notably the most osteoinductive ones, the bone morphogenetic proteins (BMPs).

中文翻译：

---

脂肪来源基质细胞的成骨分化：从实验室到临床。

除了间充质干细胞外，脂肪来源的干/基质细胞 (ASC) 是多种基于细胞的疗法的有吸引力的来源。由于它们具有在骨细胞中分化的能力，因此它们最重要的潜在应用之一与骨组织的再生有关。然而，这需要适当控制它们的成骨分化，这不仅取决于收获细胞的初始特征，还取决于用于培养的条件。在这篇综述中，我们首先简要描述了使用 ASCs 进行骨再生的临床前和临床试验，并介绍了用于表征 ASCs 成骨分化的定量参数。然后我们关注影响ACS成骨分化的可溶性因素，