Multiple nuclear localization sequences in SRSF4 protein.,Genes to Cells

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Multiple nuclear localization sequences in SRSF4 protein.
Genes to Cells ( IF 1.3 ) Pub Date : 2020-02-25 , DOI: 10.1111/gtc.12756
So Masaki _{1,

2} , Takafumi Kabuto ₃ , Kenji Suzuki ₂ , Naoyuki Kataoka _{1,

3,

4}

Affiliation

SRSF4 is one of the members of serine-/arginine (SR)-rich protein family involved in both constitutive and alternative splicing. SRSF4 is localized in the nucleus with speckled pattern, but its nuclear localization signal was not determined. Here, we have identified nuclear localization signals (NLSs) of SRSF4 by using a pyruvate kinase fusion system. As expected, arginine-/serine (RS)-rich domain of SRSF4 confers nuclear localization activity when it is fused to PK protein. We then further delineated the minimum sequences for nuclear localization in RS domain of SRSF4. Surprisingly, RS-rich region does not always have a nuclear localization activity. In addition, basic amino acid stretches that resemble to classical-type NLSs were identified. These results strongly suggest that SRSF4 protein uses two different nuclear import pathways with multiple NLSs in RS domain.

中文翻译：

SRSF4蛋白中的多个核定位序列。

SRSF4是富含丝氨酸/精氨酸（SR）的蛋白家族成员之一，参与组成型和替代剪接。SRSF4以斑点模式定位在细胞核中，但其核定位信号尚未确定。在这里，我们已经通过使用丙酮酸激酶融合系统鉴定了SRSF4的核定位信号（NLSs）。如所预期的，当SRSF4与PK蛋白融合时，富含精氨酸/丝氨酸（RS）的域会赋予核定位活性。然后，我们进一步描述了在SRSF4的RS域中进行核定位的最小序列。令人惊讶的是，RS丰富的地区并不总是具有核定位活动。另外，鉴定出类似于经典型NLS的碱性氨基酸序列。

更新日期：2020-02-25

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