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Quantitative determination of rifampicin in aquatic products by stable isotope-dilution high liquid chromatography-tandem mass spectrometry.
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2020-02-25 , DOI: 10.1002/bmc.4810 Qin Li 1, 2, 3 , Jingyuan Cao 4 , Gang Han 1, 3 , Huan Liu 1, 3 , Jie Yan 1, 3 , Lidong Wu 1, 3 , Jincheng Li 1, 3
Biomedical Chromatography ( IF 1.8 ) Pub Date : 2020-02-25 , DOI: 10.1002/bmc.4810 Qin Li 1, 2, 3 , Jingyuan Cao 4 , Gang Han 1, 3 , Huan Liu 1, 3 , Jie Yan 1, 3 , Lidong Wu 1, 3 , Jincheng Li 1, 3
Affiliation
Rifampicin is a semi-synthetic broad-spectrum antibiotic obtained from rifamycin B. It is one of the most effective first-line antituberculosis drugs and is widely used in clinical practice. In the present study, we describe a rapid and sensitive method for the determination of rifampin in aquatic products by stable isotope-dilution high liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Samples were extracted with the acetonitrile, degreased by hexane, and then concentrated by nitrogen blowing. After separation using a C18 column with a mixture of acetonitrile and water as mobile phase, it was determined by HPLC-MS/MS using the stable isotope-dilution calibration method. The performance of our method was validated. The limit of detection was 0.25 μg kg-1 and the limit of quantification was 0.5 μg kg-1 . At the three spiked levels of 0.5, 1.0 and 5.0 μg kg-1 , the average recoveries of rifampicin in different aquatic products were between 75.28 and 107.6%, and the relative standard deviation ranged from 0.81 to 13.23%. This method was successfully applied for the determination of rifampin in different kinds of aquatic products and rifampicin residue was found in aquatic products obtained from markets in Beijing, China.
更新日期：2020-04-21
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