Some wild Zygosaccharomyces rouxii impair the quality of soy sauce through the generation of unpleasant odors induced by the formation of flor. Flor formation in Z. rouxii depends on the expression of the FLO11D gene, which is a homolog of the FLO11 gene that encodes a cell surface protein in Saccharomyces cerevisiae. FLO11 expression in S. cerevisiae is regulated by multiple pathways. To investigate the regulation of FLO11D expression in Z. rouxii, we created 13 gene knockout mutants (STE12, TEC1, HOG1, MSS11, FLO8, MSN1, MSN2/4, SKO1, TUP1, CYC8, YAK1, MIG1, and SFL1) related to those pathways and examined whether these mutants form flor. Unexpectedly, SFL1 knockout mutant could only form a very weak flor due to decreased FLO11D expression, suggesting that SFL1 acts as a potential activator of flor formation through FLO11D expression. This result is in contrast to S. cerevisiae SFL1, which acts as a repressor of FLO11 expression.

中文翻译：

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SFL1鉴定为鲁氏合子酵母花序形成的正调控剂。

一些野生Zygosaccharomyces rouxii会通过形成弗洛尔而产生令人不愉快的气味，从而破坏酱油的质量。鲁氏酵母中弗洛尔的形成取决于FLO11D基因的表达，该基因是在酿酒酵母中编码细胞表面蛋白的FLO11基因的同源物。酿酒酵母中的FLO11表达受多种途径调控。为了研究鲁氏沼虾中FLO11D表达的调控，我们创建了13个基因敲除突变体（STE12，TEC1，HOG1，MSS11，FLO8，MSN1，MSN2 / 4，SKO1，TUP1，CYC8，YAK1，MIG1和SFL1）这些途径，并检查这些突变体是否形成弗洛尔。出乎意料的是，由于FLO11D表达降低，SFL1敲除突变体只能形成非常弱的弗洛尔，这表明SFL1通过FLO11D表达充当了弗洛尔形成的潜在激活剂。