In this study, to obtain further information on the structural diversity of non‐methylene‐interrupted (NMI) fatty acids (FA) in the gonads of the limpet Cellana toreuma, previously unreported minor NMI FA, fractionated by argentation thin‐layer chromatography (TLC), in the ovaries were characterized by gas chromatography–mass spectrometry (GC–MS) analysis of their 3‐pyridylcarbinol derivatives. As a result, five novel NMI FA were identified as 5,14‐pentadecadienoic acid (15: 2Δ5,14), 5,9,18‐nonadecatrienoic acid (19:3Δ5,9,18), 5,9,20‐heneicosatrienoic acid (21:3Δ5,9,20), 7,13,20‐heneicosatrienoic acid (21:3Δ7,13,20), and 5,9,22‐tricosatrienoic acid (23:3Δ5,9,22). Possible biosynthetic pathways for these FA are also discussed in relation to structural variations of NMI FA regioisomers. This study clearly reinforces the findings of our previous works with structurally diverse NMI FA with a terminal double bond.

中文翻译：

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Limpet Cellana toreuma卵巢中带有末端双键的新型非亚甲基中断的二烯和三烯脂肪酸。

在这项研究中，以获取有关壁杉Cell纲性腺中非亚甲基中断（NMI）脂肪酸（FA）的结构多样性的更多信息卵巢中先前未报告的次要NMI FA，通过银薄层色谱（TLC）分级分离，通过气相色谱-质谱法（GC-MS）分析其3-吡啶基甲醇含量进行表征。结果，确定了五个新的NMI FA，分别为5,14-十五碳二烯酸（15：2Δ5,14），5,9,18-十九碳三烯酸（19：3Δ5,9,18），5,9,20-二十碳三烯酸酸（21：3Δ5,9,20），7,13,20-异二十碳三烯酸（21：3Δ7,13,20）和5,9,22-三异三烯酸（23：3Δ5,9,22）。还针对NMI FA区域异构体的结构变异，讨论了这些FA的可能的生物合成途径。这项研究明显加强了我们以前的研究结果，这些研究涉及具有末端双键的结构多样的NMI FA。