Background/Aims: High-risk human papillomavirus (HPV) infection is associated with different malignancies, but its role in the pathogenesis of ovarian cancer remains inconclusive. Published studies demonstrated a wide variation (0–50%) in HPV prevalence in ovarian cancer. To evaluate the contribution of detection tests to controversial results in different populations, we determined the presence of HPV DNA in Russian ovarian cancer patients using 10 different PCR-based tests. Methods: Epithelial ovarian adenocarcinomas were tested with 5 general primer sets commonly used for HPV screening of cervical and ovarian cancer and 5 HPV type-specific primers. Results: The use of a single PCR primer set resulted in a wide variation (0–29%) and an underestimation of the incidence of HPV-positive cancers. The combination of MY09/MY11 and GP5+/6+ primers in nested PCR revealed HPV DNA in 53% (18/34) of adenocarcinomas. HPV16 was found in 94% of the HPV-positive cases. In 6/6 positive cases, the active status of HPV16 was demonstrated by RT-PCR detection of E6 and E7 oncogene mRNAs. Conclusion: These findings indicate the need to employ multiple PCR-based tests to detect all HPV-positive patients. The identification of viral DNA and oncogene transcripts in cancerous tissues indicate the possible role of HPV in ovarian carcinogenesis in Russia.
Intervirology

中文翻译：

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通过不同的测试系统检测人乳头瘤病毒在卵巢癌中的流行率。

背景/目的：高危型人乳头瘤病毒（HPV）感染与不同的恶性肿瘤相关，但其在卵巢癌发病机理中的作用尚无定论。已发表的研究表明，卵巢癌中HPV的患病率差异很大（0–50％）。为了评估检测测试对不同人群中有争议的结果的贡献，我们使用10种不同的基于PCR的测试确定了俄罗斯卵巢癌患者中HPV DNA的存在。方法：使用5种通常用于宫颈癌和卵巢癌HPV筛查的通用引物组和5种HPV类型特异性引物对上皮性卵巢腺癌进行检测。结果：使用单个PCR引物组会导致变异范围很大（0–29％），并且低估了HPV阳性癌症的发生率。巢式PCR中MY09 / MY11和GP5 + / 6 +引物的组合显示53％（18/34）的腺癌中有HPV DNA。在94％的HPV阳性病例中发现了HPV16。在6/6例阳性病例中，通过RT-PCR检测E6和E7癌基因mRNA证实了HPV16的活跃状态。结论：这些发现表明需要采用多种基于PCR的检测方法来检测所有HPV阳性患者。癌组织中病毒DNA和癌基因转录物的鉴定表明HPV在俄罗斯卵巢癌发生中的可能作用。
病毒学