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Detection of herpes simplex and varicella-zoster virus from skin lesions: comparison of RT-PCR and isothermal amplification for rapid identification.
Diagnostic Microbiology and Infectious Disease ( IF 2.9 ) Pub Date : 2020-02-08 , DOI: 10.1016/j.diagmicrobio.2020.115015 Monika Jevšnik 1 , Lara Lusa 2 , Tina Uršič 1 , Urška Glinšek Biškup 1 , Miroslav Petrovec 1
Diagnostic Microbiology and Infectious Disease ( IF 2.9 ) Pub Date : 2020-02-08 , DOI: 10.1016/j.diagmicrobio.2020.115015 Monika Jevšnik 1 , Lara Lusa 2 , Tina Uršič 1 , Urška Glinšek Biškup 1 , Miroslav Petrovec 1
Affiliation
We compared 2 molecular tests for detection of herpes simplex viruses 1 and 2 (HSV-1, HSV-2) and varicella-zoster virus (VZV): real-time polymerase chain reaction (RT-PCR) (Argene, BioMerieux, France) performed on an LC480 platform (Roche Applied Science, Mannheim, Germany) and isothermal amplification using a Solana HSV1 + 2/VZV assay (Quidel Corporation Worldwide Headquarters, San Diego, CA) with helicase-dependent amplification performed by a Solana® instrument. With both methods, HSV-1 was detected in 68/291 (23.4%), HSV-2 in 23/291 (7.9%), and VZV in 48/291 (16.5%) skin lesions. Both methods agreed completely only in detection of HSV-2 (kappa = 1). Concordance between Solana HSV1 + 2/VZV and RT-PCR was 98.3% (kappa = 0.95) for HSV-1 and 99.3% (kappa = 0.98) for VZV. Rapid detection of HSV-1, HSV-2, and VZV using the Solana platform is a useful method for routine diagnostics and for urgent swab samples requiring a short turnaround time.
中文翻译：
从皮肤病变中检测单纯疱疹和水痘带状疱疹病毒：RT-PCR和等温扩增的比较，可快速鉴定。
我们比较了两种用于检测单纯疱疹病毒1和2（HSV-1，HSV-2）和水痘带状疱疹病毒（VZV）的分子测试：实时聚合酶链反应（RT-PCR）（Argene，BioMerieux，法国）在LC480平台（德国曼海姆，罗氏应用科学公司）上进行电泳，并使用Solana HSV1 + 2 / VZV分析（加利福尼亚州圣地亚哥的Quidel Corporation全球总部）进行等温扩增，并通过Solana®仪器进行解旋酶依赖性扩增。两种方法均在68/291（23.4％）的HSV-1、23 / 291（7.9％）的HSV-2和48/291（16.5％）的皮肤病变中检测到VZV。两种方法仅在检测HSV-2（kappa = 1）时完全一致。Solana HSV1 + 2 / VZV与RT-PCR之间的一致性对于HSV-1为98.3％（kappa = 0.95），对于VZV为99.3％（kappa = 0.98）。快速检测HSV-1，HSV-2，
更新日期：2020-04-20
中文翻译：
从皮肤病变中检测单纯疱疹和水痘带状疱疹病毒：RT-PCR和等温扩增的比较，可快速鉴定。
我们比较了两种用于检测单纯疱疹病毒1和2（HSV-1，HSV-2）和水痘带状疱疹病毒（VZV）的分子测试：实时聚合酶链反应（RT-PCR）（Argene，BioMerieux，法国）在LC480平台（德国曼海姆，罗氏应用科学公司）上进行电泳，并使用Solana HSV1 + 2 / VZV分析（加利福尼亚州圣地亚哥的Quidel Corporation全球总部）进行等温扩增，并通过Solana®仪器进行解旋酶依赖性扩增。两种方法均在68/291（23.4％）的HSV-1、23 / 291（7.9％）的HSV-2和48/291（16.5％）的皮肤病变中检测到VZV。两种方法仅在检测HSV-2（kappa = 1）时完全一致。Solana HSV1 + 2 / VZV与RT-PCR之间的一致性对于HSV-1为98.3％（kappa = 0.95），对于VZV为99.3％（kappa = 0.98）。快速检测HSV-1，HSV-2，
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