Because broad genetic diversity has recently been detected in Torque teno sus viruses (TTSuV1 and TTSuVk2), the viral genome detection method needs to be improved to understand the prevalence of these viruses. Here, we established single PCR‐based detection methods for the TTSuV1 and TTSuVk2a genomes with newly designed primer pairs and applied them to investigate the prevalence of TTSuV1 and TTSuVk2a in Japanese pig populations. The results revealed that 98.2% and 81.7% of the pig farms tested positive for the TTSuV1 and TTSuVk2a genomes, respectively, indicating that both TTSuV1 and TTSuVk2a are widespread in Japan.

中文翻译：

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在日本的猪群中，全国性的Teno Tens sus病毒1和k2a流行。

由于最近已在扭矩Tens sus病毒（TTSuV1和TTSuVk2）中检测到广泛的遗传多样性，因此需要改进病毒基因组检测方法以了解这些病毒的流行情况。在这里，我们使用新设计的引物对建立了针对TTSuV1和TTSuVk2a基因组的基于PCR的单一检测方法，并将其应用于日本猪种群中TTSuV1和TTSuVk2a的流行情况。结果显示，分别有98.2％和81.7％的猪场测试TTSuV1和TTSuVk2a基因组呈阳性，表明TTSuV1和TTSuVk2a在日本都很普遍。