BACKGROUND Rheumatoid arthritis (RA) is a systemic autoimmune disease characterized by chronic and progressive inflammation that can cause a high degree of disability in affected individuals. Proinflammatory cytokines play central roles in the development of degradative and inflammatory responses in RA. IL-29 has been identified in RA and reported as a biomarker of the disease. OBJECTIVE To analyze serum levels and accuracy of IL-29 in RA patients compared to healthy subjects and patients with other rheumatic diseases. METHODS IL-29 serum levels were measured in 121 patients with RA, 53 patients with systemic lupus erythematosus (SLE), 60 patients with systemic sclerosis (SSc), 29 patients with fibromyalgia (FM), 50 patients with osteoarthritis (OA) and 68 healthy individuals as controls. IL-29 levels in serum were investigated by ELISA. Sensitivity, specificity and likelihood ratios (LR) for having RA were calculated. RESULTS Serum levels of IL-29 were increased in RA patients 113.6 (IQR = 31.25-308.5) pg/ml compared to non-RA patients (SLE, SSc, OA, and FM) (31.25 pg/ml) and healthy controls (31.25 pg/ml, p < 0.001). The IL-29 cut-off values to distinguish patients with RA from non-RA patients were 61.11 pg/ml (sensitivity 57.02, specificity 92.71, LR: 7.82) and for all subjects 32.96 pg/ml (sensitivity 64.46, specificity 87.31, LR: 5.08). Additionally, IL-29 correlated negatively with age (r=-0189, p = 0.038) and disease duration (-0.192, p = 0.037). Interestingly, IL-29 correlated positively with neutrophil count in RA patients positive for rheumatoid factor (r = 0.259, p = 0.022). CONCLUSION IL-29 is higher in the serum of patients with RA compared to non-RA subjects and may have potential for use as a biological marker.

中文翻译：

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白细胞介素 29 在类风湿关节炎和其他风湿性疾病患者中的敏感性和特异性。


背景类风湿性关节炎(RA)是一种全身性自身免疫性疾病，其特征是慢性进行性炎症，可导致受影响个体的高度残疾。促炎细胞因子在 RA 退化和炎症反应的发展中发挥着核心作用。 IL-29 已在 RA 中被发现，并被报道为该疾病的生物标志物。目的 分析 RA 患者与健康受试者和其他风湿病患者的血清 IL-29 水平和准确性。方法 测定 121 例 RA 患者、53 例系统性红斑狼疮 (SLE) 患者、60 例系统性硬化症 (SSc) 患者、29 例纤维肌痛 (FM) 患者、50 例骨关节炎 (OA) 患者和 68 例患者的血清 IL-29 水平。健康个体作为对照。通过 ELISA 研究血清中的 IL-29 水平。计算了 RA 的敏感性、特异性和似然比 (LR)。结果 与非 RA 患者（SLE、SSc、OA 和 FM）（31.25 pg/ml）和健康对照（31.25皮克/毫升，p < 0.001）。区分 RA 患者和非 RA 患者的 IL-29 临界值为 61.11 pg/ml（敏感性 57.02，特异性 92.71，LR：7.82），所有受试者的 IL-29 临界值为 32.96 pg/ml（敏感性 64.46，特异性 87.31，LR） ：5.08）。此外，IL-29 与年龄 (r=-0189，p = 0.038) 和疾病持续时间 (-0.192，p = 0.037) 呈负相关。有趣的是，在类风湿因子阳性的 RA 患者中，IL-29 与中性粒细胞计数呈正相关（r = 0.259，p = 0.022）。结论 与非 RA 受试者相比，RA 患者血清中的 IL-29 含量较高，并且可能具有用作生物标志物的潜力。