The implementation of self-assembled biomolecules on solid materials, in particular, sensor and electrode surfaces, gains increasing importance for the design of stable functional platforms, bioinspired materials, and biosensors. The present study reports on the formation of a planar hybrid lipid/polymer membrane on a crystalline surface layer protein (SLP) lattice. The latter acts as a connecting layer linking the biomolecules to the inorganic base plate. In this approach, chemically bound lipids provided hydrophobic anchoring moieties for the hybrid lipid/polymer membrane on the recrystallized SLP lattice. The rapid solvent exchange technique was the method of choice to generate the planar hybrid lipid/polymer membrane on the SLP lattice. The formation process and completeness of the latter were investigated by quartz crystal microbalance with dissipation monitoring and by an enzymatic assay using the protease subtilisin A, respectively. The present data provide evidence for the formation of a hybrid lipid/polymer membrane on an S-layer lattice with a diblock copolymer content of 30%. The hybrid lipid/polymer showed a higher stiffness compared to the pure lipid bilayer. Most interestingly, both the pure and hybrid membrane prevented the proteolytic degradation of the underlying S-layer protein by the action of subtilisin A. Hence, these results provide evidence for the formation of defect-free membranes anchored to the S-layer lattice.

中文翻译：

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结晶表面层蛋白晶格上混合脂质/聚合物膜的形成和特征。

在固体材料（特别是传感器和电极表面）上实现自组装生物分子对于稳定功能平台、仿生材料和生物传感器的设计变得越来越重要。本研究报告了在结晶表面层蛋白（SLP）晶格上形成平面杂化脂质/聚合物膜。后者充当连接层，将生物分子连接到无机基板。在这种方法中，化学结合的脂质为重结晶的SLP晶格上的杂化脂质/聚合物膜提供疏水性锚定部分。快速溶剂交换技术是在 SLP 晶格上生成平面杂化脂质/聚合物膜的选择方法。后者的形成过程和完整性分别通过具有耗散监测的石英晶体微天平和使用蛋白酶枯草杆菌蛋白酶A的酶测定来研究。目前的数据为在二嵌段共聚物含量为 30% 的 S 层晶格上形成杂化脂质/聚合物膜提供了证据。与纯脂质双层相比，杂化脂质/聚合物表现出更高的刚度。最有趣的是，纯膜和混合膜都通过枯草杆菌蛋白酶 A 的作用阻止了底层 S 层蛋白的蛋白水解降解。因此，这些结果为锚定到 S 层晶格的无缺陷膜的形成提供了证据。