Recurrent spontaneous abortion (RSA) is the most common pregnancy related complication, affecting 1-5 % of pregnancies. Despite hormonal, genetic and anatomical factors that result in abortion, impairment of immune response at the feto-maternal interface during the first trimester of pregnancy is also one of the main causes of RSA. In the present study, we evaluated the frequency of blood and uterine group 2 innate lymphoid cells (ILC2s), their subsets and regulatory T cells (Tregs) in CBA/J × DBA/2 J as an abortion-prone model compared to normal pregnant (NP) mice using immunophenotyping. Results indicated that the percentages of ILC2s were significantly decreased in the AP group compared to the NP group at mid-gestation (P ≤ 0.01). Moreover, the percentages of both blood and uterine nILC2s were increased in NP mice at mid-gestation (P ≤ 0.01, and P ≤ 0.05, respectively), while iILC2s significantly increased in AP mice at mid-gestation (P ≤ 0.01, and P ≤ 0.05, respectively). Tregs were reduced in AP mice at both early and mid-gestation stages (P ≤ 0.01). Overall, our findings suggest that the changes in blood and uterine ILC2s might be associated with abortion in mice.

中文翻译：

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易流产小鼠中2型先天淋巴样细胞和调节性T细胞的频率分析。

反复自然流产（RSA）是最常见的妊娠相关并发症，影响1-5％的怀孕。尽管有激素，遗传和解剖学因素导致流产，但在怀孕的头三个月期间，胎儿-母亲界面的免疫反应受到损害也是RSA的主要原因之一。在本研究中，我们评估了血液和子宫第2组先天性淋巴样细胞（ILC2s）的频率，其子集和调节性T细胞（Tregs）在CBA / J×DBA / 2 J中作为与正常妊娠相比易流产的模型的频率（NP）小鼠使用免疫表型。结果表明，在妊娠中期，AP组的ILC2s百分比显着低于NP组（P≤0.01）。此外，妊娠中期NP小鼠的血液和子宫nILC2s的百分比均升高（P≤0.01，和P≤0.05），而iILC2s在妊娠中期的AP小鼠中显着增加（分别为P≤0.01和P≤0.05）。在妊娠早期和中期，AP小鼠的Treg均降低（P≤0.01）。总体而言，我们的发现表明血液和子宫ILC2的变化可能与小鼠流产有关。