A sensitive, rapid and cost-effective method based on HPTLC with UV detection was developed for the quantitation of Glibenclamide (GLIBEN), Rosiglitazone maleate (ROSI) and Metformin hydrochloride (MET) from a combined dosage form. Pre-coated RP-18 F254s aluminum sheets were used as the stationary phase. Methanol-tetrahydrofuran-water-glacial acetic acid (16: 3.6: 4: 0.4, v/v) used as the mobile phase, along with chamber saturation of 10 min offered an optimum migration (Rf = 0.54, 0.62 and 0.80 for GLIBEN, ROSI and MET, respectively). TLC Scanner 3 was used for densitometric evaluation of the chromatograms. DigiStore 2 Documentation System with winCATS software version 1.4.10 was used for the quantitation and photodocumentation. The LOD for GLIBEN, ROSI and MET was found to be 80 ng, 80 ng and 48 ng, respectively. Moreover, the LOQ was 200 ng, 200 ng and 120 ng for GLIBEN, ROSI and MET, respectively. The method was linear for GLIBEN (r = 0.9991), ROSI (r = 0.9993) and MET (r = 0.9988) within the tested range (200-1000, 200-1000 and 120-600 ng/band, respectively). The method was found to be precise and accurate for all the three drugs. The method was applied for the analysis of Triglucored tablets, and it proved to be a reliable quality control tool for the routine analysis of GLIBEN, ROSI and MET in a combined dosage form.

中文翻译：

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从多剂量剂型估算格列本脲，马来酸罗格列酮和盐酸二甲双胍的灵敏的HPTLC方法。

开发了一种基于HPTLC和紫外检测的灵敏，快速且经济高效的方法，用于从联合剂型中定量测定格列本脲（GLIBEN），马来酸罗格列酮（ROSI）和盐酸二甲双胍（MET）。预涂的RP-18 F254s铝板用作固定相。甲醇-四氢呋喃-水-冰醋酸（16：3.6：4：0.4，v / v）用作流动相，加上10分钟的室饱和度，GLIBEN的最佳迁移率（Rf = 0.54，0.62和0.80， ROSI和MET）。TLC扫描仪3用于色谱的光密度测定。定量和照片记录使用具有winCATS软件1.4.10版本的DigiStore 2文档系统。发现GLIBEN，ROSI和MET的LOD分别为80 ng，80 ng和48 ng。此外，最低定量限为200 ng，GLIBEN，ROSI和MET分别为200 ng和120 ng。对于GLIBEN（r = 0.9991），ROSI（r = 0.9993）和MET（r = 0.9988）在测试范围内（分别为200-1000、200-1000和120-600 ng / band），该方法是线性的。发现该方法对于所有三种药物都是精确的。该方法用于甘油三酸酯的分析，被证明是常规剂量的GLIBEN，ROSI和MET常规分析的可靠质量控制工具。