Innovative therapies combining gene-corrected stem cells and the production of bioengineered tissues to treat epidermolysis bullosa are emerging. However, quantitative tests to measure the adhesion forces between two highly viscoelastic substrates such as those found in bilayered bioengineered skin are needed and are still lacking. The objective of this study was to develop a mechanical test to measure the dermal-epidermal adhesion strength of our bilayered tissue-engineered skin substitute (TES) produced with the self-assembly method. We developed a peel test, which allows the displacement of both skin layers in a T configuration, based on the ASTM International standard. A MATLAB program was written to process and analyze raw data. The experimental setup was tested by measuring the dermal-epidermal adhesion strength in TESs produced with normal or collagen VII-deficient cells. Our peel testing method allowed us to detect the impact of the absence of collagen VII in the dermal-epidermal adhesion strength of TESs and also to examine the progression of the dermal-epidermal adhesion strength in relation to culture time in normal TES. Impact statement This study describes a method for assessing the adhesion strength at the dermal-epidermal junction of individual tissue-engineered skin substitute (TES). An ASTM standardized protocol of peel testing was designed to measure this important mechanical property. Our innovative approach will serve as a quality control in the production, improvement, and application of TESs for the treatment of pathologies affecting the dermal-epidermal adhesion such as epidermolysis bullosa. Data presented contribute to research on the interfaces between biological substrates and provide a reference factor for the characterization of products derived from tissue engineering.

中文翻译：

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剥离测试，以评估组织工程皮肤中皮肤表皮连接点的粘附强度。

结合基因校正的干细胞和生物工程组织的生产来治疗大疱表皮松解的创新疗法正在兴起。但是，仍然需要定量测试来测量两种高粘弹性底物（例如在双层生物工程皮肤中发现的底物）之间的粘合力。这项研究的目的是开发一种机械测试，以测量通过自组装方法生产的双层组织工程皮肤替代品（TES）的皮肤-表皮粘合强度。我们根据ASTM国际标准开发了一项剥离测试，该测试允许T形配置中的两个蒙皮层均发生位移。编写了一个MATLAB程序来处理和分析原始数据。通过测量由正常或缺乏VII胶原蛋白的细胞产生的TES中的真皮-表皮粘合强度来测试实验设置。我们的果皮测试方法使我们能够检测到胶原蛋白VII的缺失对TESs的表皮粘合强度的影响，并且可以检查正常TES中表皮-粘合强度与培养时间的关系。影响陈述这项研​​究描述了一种评估单个组织工程化皮肤替代品（TES）在真皮与表皮交界处的粘合强度的方法。设计了ASTM剥离测试标准化协议来测量这一重要的机械性能。我们的创新方法将在生产，改进，TES的应用和在治疗影响真皮-表皮黏附的病理如大疱表皮松解中的应用。所提供的数据有助于研究生物底物之间的界面，并为表征源自组织工程的产品提供了参考因素。