Improving the proliferation of probiotics (ca. Bifidobacterium) and inhibiting the growth of pathogenic bacteria (ca. Escherichia coli) is crucial for human health. This study demonstrates the fabrication of core-shell structure fibers using electrohydrodynamic (EHD) 3D printing to help improve gastrointestinal tract microbial content. These fibers have various geometries and are capable of encapsulating stachyose into cellulose acetate (shell layer) and loading proteoglycan into polyacrylic resin II (core layer). The impact of membrane geometry on drug release behavior and the effect of exchanging the loading site on physicochemical properties of the resulting fibers were studied. The printed fibrous membranes possess a biphasic drug release profile in simulated intestinal fluid with a burst release within the first 12 hours and a slower sustained release up to 72 hours. The speed order priority for drug release rate of the printed membrane was whole-circle > semi-circle > square. Moreover, the membranes exhibit good biocompatibility on L929 cells and excellent improvement effects on Bifidobacterium bifidum, combining inhibition effects on Escherichia coli. In summary, the dual-drug fibrous membranes presented here and their precision-fabricated patterns pave a new direction for improving the gastrointestinal tract microbial eco-system health in the human body.

中文翻译：

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使用3D打印改善肠胃道微生物健康的核壳多药平台。

改善益生菌（大约双歧杆菌）的增殖并抑制病原菌（大约大肠杆菌）的生长对于人类健康至关重要。这项研究演示了使用电液动力学（EHD）3D打印制造核-壳结构纤维，以帮助提高胃肠道微生物含量。这些纤维具有各种几何形状，并且能够将水苏糖包封到乙酸纤维素（壳层）中并且将蛋白聚糖装载到聚丙烯酸树脂II（芯层）中。研究了膜几何形状对药物释放行为的影响以及交换负载位点对所得纤维理化性质的影响。印刷的纤维膜在模拟的肠液中具有双相药物释放曲线，在最初的12小时内爆发释放，而在72小时内持续释放较慢。所印刷的膜的药物释放速率的速度顺序优先顺序为整圆>半圆>正方形。而且，该膜对L929细胞表现出良好的生物相容性，并且对双歧双歧杆菌具有优异的改善效果，同时结合了对大肠杆菌的抑制效果。综上所述，本文介绍的双药纤维膜及其精细制作的图案为改善人体胃肠道微生物生态系统健康铺平了新的方向。该膜对L929细胞表现出良好的生物相容性，对双歧双歧杆菌具有优异的改良效果，同时兼具对大肠杆菌的抑制作用。综上所述，本文介绍的双药纤维膜及其精细制作的图案为改善人体胃肠道微生物生态系统健康铺平了新的方向。该膜对L929细胞表现出良好的生物相容性，对双歧双歧杆菌具有优异的改良效果，同时兼具对大肠杆菌的抑制作用。综上所述，本文介绍的双药纤维膜及其精细制作的图案为改善人体胃肠道微生物生态系统健康铺平了新的方向。