Amblyomma sculptum is a tick of medical-veterinary importance. Areas with high infestations need to be monitored, and parasitized hosts treated accordingly. Carbon dioxide (CO2) traps and acaricides are commonly deployed as control measures, although with some disadvantages such as high costs, challenging logistics and vertebrate intoxication. Semiochemicals have the potential to improve tick attraction to traps and monitoring devices and alleviate the burden of A. sculptum infestations. Four concentrations (10, 5, 2.5 and 1.25 %) of 13 semiochemical candidates (CO2 only at 5 % as the standard, benzaldehyde, benzoic acid, salicylic acid, 2,6 diclorophenol, R-limonene, S-limonene, methyl salicylate, 1-octen-3-ol, acetone, ammonium hydroxide, isobutyric acid and lactic acid) were tested on unfed A. sculptum nymphs and adults using a Y-tube olfactometer to evaluated repellence and attraction behaviors. All stages tested were attracted to CO2, whereas nymphs were repelled by benzaldehyde and R-limonene, both at 10 %, and isobutyric acid at 5 and 10 %. Nymphs were attracted by methyl salicylate, benzoic acid and salicylic acid, all at 1.25 %, and by ammonium hydroxide at 2.5 %. Males were attracted by benzoic acid at 2.5 %, while females were repelled by benzaldehyde at 5 %. Mixtures with the attractive compounds achieved no attraction response. The compounds that caused attractiveness in the olfactometer assay (CO2, methyl salicylate, benzoic acid, salicylic acid and ammonium hydroxide) were placed randomly in traps in a grassland plot naturally infested with A. sculptum in triplicate. Notably, dry ice (CO2) remained the best at luring ticks in the field (P < 0.001). Benzoic acid should be further investigated since attractant activity was strongly confirmed in both laboratory and field tests. On the other hand, isobutyric acid and R-limonene could be better exploited due to their repellent role revealed by the lab assay, which makes them worthwhile molecules as natural repellents for the management of this tick.

中文翻译：

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吸引或排斥浮雕雕刻tick：筛选化学信息素。

胸膜浮雕是医学兽医重要性的象征。需要监测感染高发地区，并应对寄生虫寄主。二氧化碳（CO2）捕集阱和杀螨剂通常被用作控制措施，尽管有一些缺点，例如成本高，物流困难和脊椎动物中毒。Semiochemicals有潜力改善tick对诱捕器和监测设备的吸引力，并减轻A. sculptum侵扰的负担。四种浓度（10％，5％，2.5％和1.25％）的13种化学信息素候选物（CO2仅以5％的标准浓度），苯甲醛，苯甲酸，水杨酸，2,6二氯苯酚，R-柠檬烯，S-柠檬烯，水杨酸甲酯，在未进料的A上测试了1-辛烯-3-醇，丙酮，氢氧化铵，异丁酸和乳酸）。雕塑若虫和成虫使用Y型管嗅觉仪评估驱蚊和吸引行为。测试的所有阶段均被CO2吸引，而若虫则被10％的苯甲醛和R-柠檬烯以及5％和10％的异丁酸所排斥。若虫被水杨酸甲酯，苯甲酸和水杨酸（浓度均为1.25％）和氢氧化铵（浓度为2.5％）所吸引。雄性被2.5％的苯甲酸吸引，而雌性被5％的苯甲醛排斥。与有吸引力的化合物的混合物没有达到吸引反应。在嗅觉测定法中引起吸引力的化合物（CO2，水杨酸甲酯，苯甲酸，水杨酸和氢氧化铵）被随机放置在自然覆盖草皮一式三份的草地上的陷阱中。值得注意的是 干冰（CO2）仍是田间引诱tick虫的最佳方式（P <0.001）。苯甲酸应进一步研究，因为在实验室和现场测试中都强烈证实了引诱剂的活性。另一方面，由于实验室测定法揭示了异丁酸和R-柠檬烯的驱避作用，因此可以更好地利用异丁酸和R-柠檬烯，这使它们成为有价值的分子，可以作为天然驱蚊剂来控制这种虱子。